Inhibition of TNFα-induced interleukin-6 gene expression by barley (Hordeum vulgare) ethanol extract in BV-2 microglia

Genes & Genomics
Jihye ChoiYoung Han Lee

Abstract

Inflammation in the central nervous system is closely associated with pathological neurodegenerative diseases as well as psychiatric disorders. Prolonged activation of microglia can produce many inflammatory mediators, which may result in pathological neurotoxic side effects. Interleukin (IL)-6 serves as a hallmark of the injured brain. Whole grains are known to contain many bioactive components. However, little information is available about anti-neuroinflammatory effects of grains in the CNS. This study aims to investigate the effect of Hordeum vulgare ethanol extract (HVE) on the suppression of IL-6 expression in BV2 microglia. Inhibitory effects of HVE on IL-6 expression were analyzed by immunoblot anaysis, immunofluoresce microscopic analysis, reverse transcription-polymerase chain reaction, and luciferase promoter reporter assay. HVE inhibited TNFα-induced phosphorylation of IKKα/β, IκB, and p65/RelA NF-κB. TNFα-induced IL-6 mRNA expression and promoter activity were reduced by HVE. Point mutation of NF-κB-binding site within the IL-6 gene promoter abolished TNFα-induced reporter activity, whereas exogenous expression of p65 NF-κB enhanced IL-6 promoter activity. NF-κB-binding site within the IL-6 promoter region is a HVE...Continue Reading

References

May 1, 1990·Molecular and Cellular Biology·T A Libermann, D Baltimore
Apr 10, 1997·The New England Journal of Medicine·P J Barnes, M Karin
Jun 1, 1997·Trends in Neurosciences·L A O'Neill, C Kaltschmidt
Oct 16, 1999·The Journal of Biological Chemistry·H SakuraiW Toriumi
Jan 3, 2001·The Journal of Clinical Investigation·P P Tak, G S Firestein
Feb 13, 2001·The Journal of Clinical Investigation·M P Mattson, S Camandola
Oct 16, 2001·Journal of Agricultural and Food Chemistry·D HernanzC Gómez-Cordovés
Oct 3, 2002·Nature Reviews. Immunology·Qiutang Li, Inder M Verma
Oct 16, 2002·Glia·P EikelenboomA Williams
Mar 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mekhine BaccamGail A Bishop
May 4, 2004·Nature Reviews. Molecular Cell Biology·Lin-Feng Chen, Warner C Greene
Apr 19, 2005·Journal of Neuroimmunology·Reiko KunoAkio Suzumura
May 14, 2005·Current Opinion in Neurology·Luisa Minghetti
Oct 4, 2005·The Journal of Clinical Investigation·Jun-Li LuoMichael Karin
Dec 21, 2006·Journal of Agricultural and Food Chemistry·Zory Quinde-Axtell, Byung-Kee Baik
Mar 14, 2007·Current Pharmaceutical Design·Takashi OkamotoKaori Asamitsu
Feb 21, 2009·Journal of Agricultural and Food Chemistry·Federico FerreresPaula B Andrade
Apr 4, 2009·Nature Reviews. Cancer·Frances Balkwill
May 23, 2009·Nature Reviews. Immunology·Serge Rivest
Jun 13, 2009·Recent Patents on CNS Drug Discovery·Alan J Nimmo, Robert Vink
Feb 27, 2010·Biological Psychiatry·Nikoletta DobosUlrich L M Eisel
Mar 20, 2010·Critical Reviews in Food Science and Nutrition·Neal Okarter, Rui Hai Liu
Aug 7, 2013·Molecular Cancer·Bastian Hoesel, Johannes A Schmid
Feb 14, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Iain L CampbellJuan Hidalgo
Sep 6, 2014·Cold Spring Harbor Perspectives in Biology·Toshio TanakaTadamitsu Kishimoto
Mar 10, 2015·Current Opinion in Immunology·Christoph GarbersStefan Rose-John
Jun 27, 2015·Progress in Neuro-psychopharmacology & Biological Psychiatry·Yong-Ku KimBrian E Leonard
Oct 16, 2015·Critical Reviews in Food Science and Nutrition·Arashdeep Singh, Savita Sharma
Nov 17, 2015·Expert Opinion on Therapeutic Targets·Emiliano Peña-AltamiraBarbara Monti
Jan 7, 2016·Frontiers in Molecular Neuroscience·Ruey-Horng ShihChuen-Mao Yang
Mar 24, 2016·Trends in Cell Biology·Jeffrey L Frost, Dorothy P Schafer
Mar 27, 2016·Biochimica Et Biophysica Acta·Michelle RothaugStefan Rose-John
Dec 6, 2016·Nature Reviews. Immunology·Burkhard BecherJoan Goverman
Jul 8, 2017·Journal of Medicinal Food·Soon Young ShinYoung Han Lee
Sep 26, 2017·Frontiers in Cellular Neuroscience·Gaurav Singhal, Bernhard T Baune

❮ Previous
Next ❯

Citations

Feb 8, 2020·Current Neuropharmacology·Shareen Singh, Thakur Gurjeet Singh
May 18, 2020·Current Drug Discovery Technologies·Mohsen Haghir EbrahimabadiLaila Shirbeigi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.