Inhibition of Toxoplasma gondii replication in IFN-gamma-activated human intestinal epithelial cells

Immunology and Cell Biology
I H Dimier, D T Bout

Abstract

Toxoplasma gondii is an obligate intracellular parasite which is responsible for severe disease after congenital infection and in immunocompromised patients, for which there is no effective therapy. In acquired toxoplasmosis, T. gondii first invade enterocytes and are disseminated throughout the body. Treatment of the adherent human intestinal cell line CaCO2 with recombinant human IFN-gamma inhibited the replication of T. gondii. Growth of the parasite was measured in vitro by [3H]-uracil incorporation assays 18 h after infection. This assay showed that when cells were pretreated with IFN-gamma concentrations ranging from 2.5 to 5000 U/ml, a high degree of inhibition of T. gondii replication could be observed, with the effect being dose dependent. This could be of relevance as a first line of defence against human Toxoplasma infection. Inhibition is due to a different mechanism from that existing in mouse macrophages and human fibroblasts: L-arginine-dependent production of reactive nitrogen intermediates, reactive oxygen intermediates synthesis or production of indoleamine 2.3 dioxygenase were not responsible for inhibition of T. gondii proliferation.

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Citations

Jan 13, 2000·Immunobiology·O Liesenfeld
Jan 13, 2000·Immunobiology·D BoutD B Gatel
Mar 1, 2016·Neuropharmacology·Nicholas H HuntHelen J Ball
Mar 23, 2005·Microbiology and Immunology·Lian-Xun PiaoAkihiko Yano
Feb 1, 2005·Parasite Immunology·A Abou-BacarE Candolfi
Sep 22, 2005·Immunology and Cell Biology·Alexander W PfaffErmanno Candolfi
Jul 3, 2016·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Marisol Pallete BriceñoNeide Maria Silva
Apr 14, 2017·Clinical Microbiology Reviews·Mohamed-Ali HakimiL David Sibley
Feb 25, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jun OhshimaMasahiro Yamamoto
Jul 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·L C Gavrilescu, E Y Denkers

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