PMID: 9416416Jan 7, 1998Paper

Inhibition of transforming growth factor alpha stimulation of human squamous cell carcinoma of the head and neck with anti-TGF-alpha antibodies and tyrphostin

Annals of Surgical Oncology
C C SolorzanoS L MacKay

Abstract

Transforming growth factor alpha (TGF-alpha) and its receptor (EGF-R) may regulate normal and malignant epithelial cell growth by an autocrine mechanism. We investigated the role of TGF-alpha in regulating head and neck SCC tumor growth. TGF-alpha and EGF-R levels were measured in 7 SCC cell lines and 14 SCC biopsies by RIA, Scatchard, and Western analysis. TGF-alpha autocrine stimulation of DNA synthesis in SCC cell lines was assessed by incubation with TGF-alpha neutralizing antibodies and tyrphostin AG 1478, a selective and potent inhibitor of EGF-R kinase. All SCC cell lines synthesized TGF-alpha and expressed elevated EGF-R levels compared to normal keratinocytes. Twelve of the 14 SCC biopsies contained TGF-alpha protein and 8 had specific EGF-R. Exogenous TGF-alpha or EGF significantly increased DNA synthesis in 4 of 5 SCC cell lines. TGF-alpha neutralizing antibodies or tyrphostin AG 1478 reduced DNA synthesis in the two SCC cell lines (FaDu and SCC9) tested. These results indicate that SCC cell lines and tumors usually synthesize TGF-alpha, have elevated levels of EGF-R, and are mitogenically stimulated by a TGF-alpha autocrine system. Selective inhibition of the TGF-alpha system by EGF-R kinase inhibitors or TGF-alpha ...Continue Reading

References

Jan 1, 1992·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·M E ChristensenP Bretlau
Jan 1, 1992·The Journal of Pathology·N R LemoineW J Gullick
Feb 1, 1991·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J A BarnesM D Hollenberg
Jan 15, 1990·International Journal of Cancer. Journal International Du Cancer·K YoshidaE Tahara
Dec 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C B SiegallI Pastan
Aug 1, 1989·Carcinogenesis·R ToddD T Wong
Aug 26, 1988·Cell·R Derynck
Nov 1, 1994·European Journal of Biochemistry·N Osherov, A Levitzki
Feb 6, 1995·Biochemical and Biophysical Research Communications·H Modjtahedi, C Dean
Mar 24, 1995·Science·A Levitzki, A Gazit
Jan 1, 1994·Breast Cancer Research and Treatment·T Rajkumar, W J Gullick
Feb 9, 1996·The Journal of Biological Chemistry·J BaselgaA Pandiella

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.