PMID: 2498916Jan 1, 1989Paper

Inhibition of transient gene expression in Chinese hamster ovary cells by cyclobutane dimers and (6-4) photoproducts in transfected ultraviolet-irradiated plasmid DNA

Plasmid
D L MitchellR S Nairn

Abstract

Using a transient gene expression assay to measure host cell reactivation, the effects of cyclobutane dimer and noncyclobutane dimer uv photoproducts on expression of a reporter gene were examined in normal and repair-deficient Chinese hamster ovary (CHO) cell lines. Ultraviolet damage in plasmid pRSV beta gal DNA, containing the Escherichia coli beta-galactosidase gene, resulted in reduced reporter gene expression in both uv-hypersensitive mutant CHO cell lines UV5 and UV61 relative to wild-type, parental AA8 cells. However, the effects of uv irradiation of transfected plasmid DNA on gene activity were reduced in UV61, a mutant with normal (6-4) photoproduct repair, compared to UV5, which is deficient in (6-4) photoproduct repair; this reduction correlated with the intermediate uv-hypersensitivity of UV61. Selective removal of cyclobutane dimers by in vitro photoreactivation of uv-irradiated plasmid DNA prior to transfection substantially increased reporter gene activity in both uv-hypersensitive mutant cell lines. This increase was significantly greater in UV61 than in UV5, consistent with UV5 being deficient in repair of both (6-4) photoproducts and cyclobutane dimers. These results suggest that unrepaired (6-4) photoproduct...Continue Reading

References

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Citations

Jul 1, 1995·Plant Physiology·A B Britt
Sep 1, 1994·Photochemistry and Photobiology·D L MitchellL H Lutze
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Anne B. Britt
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·C Z JiangA B Britt
Jun 1, 1989·Photochemistry and Photobiology·D L Mitchell, R S Nairn
May 11, 2016·Journal of Photochemistry and Photobiology. B, Biology·C BraunU E Siebeck
Aug 18, 2017·The EMBO Journal·Pablo E García-NietoAshby J Morrison

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