Inhibition of Transient Receptor Potential Vanilloid 6 channel, elevated in human ovarian cancers, reduces tumour growth in a xenograft model

Journal of Cancer
Hui XueJohn M Stewart

Abstract

Background: Transient Receptor Potential Vanilloid 6 (TRPV6), a non-voltage gated calcium channel, is implicated in malignancies and correlates with Gleason scores in prostate cancer and with poor prognosis in breast cancer. Data on the TRPV6 status of ovarian malignancies has not received significant attention. The effect of inhibiting TRPV6 activity on ovarian tumour growth has never been reported. Methods: We quantified TRPV6 mRNA and protein in biopsies of five types of ovarian cancer at different stages and grades by quantitative PCR and immunohistochemistry respectively. We verified the presence of TRPV6 in SKOV-3 cells and xenografts by Western Blotting. NOD/SCID mice bearing xenografted ovarian tumours derived from SKOV-3 were treated daily with TRPV6-antagonistic peptides (SOR-C13 and SOR-C27) at 400, 600 and 800 mg/kg delivered intraperitoneally (i.p.) over 12 days. Data from qPCR and tumour growth experiments were compared with a Student's t-test. Immunohistochemical ranking of staining were compared with Kruskall-Wallace one-way ANOVA and Dunn's Multiple Comparison post-test. Results: TRPV6 mRNA and protein are significantly elevated at all stages and grades of 5 ovarian cancer types over normal tissue. Overall qPCR...Continue Reading

Citations

May 16, 2019·Cold Spring Harbor Perspectives in Biology·Sarah J Roberts-ThomsonGregory R Monteith
Feb 20, 2020·The Journal of Physiology·Maria V YelshanskayaAlexander I Sobolevsky
Aug 23, 2020·Cancers·Jason I E Bruce, Andrew D James
Aug 9, 2020·Frontiers in Physiology·Eszter BecskeháziViktória Venglovecz
Oct 30, 2020·Antioxidants & Redox Signaling·Huan-Ling LaiElaine Lai-Han Leung
Jun 20, 2020·Molecular Cell·Saverio MarchiPaolo Pinton
Jun 15, 2019·Cell Calcium·Sana KoubaMarie Potier-Cartereau
Jul 22, 2021·The FEBS Journal·Ling WuLiang Zhao

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Methods Mentioned

BETA
biopsies
xenografts
biopsy
electrophoresis

Software Mentioned

TissueScan
GraphPad
Prism

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