PMID: 2114171Jun 27, 1990Paper

Inhibition of transport system b0,+ in blastocysts by inorganic and organic cations yields insight into the structure of its amino acid receptor site

Biochimica Et Biophysica Acta
L J Van WinkleJ M Gorman

Abstract

The most conspicuous, Na(+)-independent amino acid transport process in preimplantation mouse blastocysts was provisionally designated system b0,+ because it accepts some cationic and zwitterionic amino acids about equally well as substrates. Although system b0,+ is not Na(+)-stimulated, it has not been determined if it is inhibited by Na+, or if its activity is affected by most other ions. Therefore, we measured uptake of amino acids by blastocysts in isotonic solutions of different ionic and nonionic osmolites. Na(+)-independent L-leucine uptake was unaffected by the ion concentration, but L-lysine transport was several-fold faster in isotonic solutions of non-electrolytes than in similar solutions of inorganic and organic salts or zwitterions. The Km value for 'Na(+)-independent' L-lysine transport was about 10-fold higher in isotonic salt solutions than in solutions of nonionic osmolites, whereas the Km value for L-leucine transport was about the same in either type of solution. Therefore, inorganic and organic cations and the cationic portions of zwitterions appear to compete with cationic but not zwitterionic amino acids for system b0,+ receptor sites. The cation, harmaline, was a particularly strong competitive inhibitor...Continue Reading

References

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Citations

Nov 1, 1990·Developmental Biology·L J Van WinkleR L Garton
Jul 1, 1991·Developmental Biology·L J Van Winkle, A L Campione
Oct 18, 2006·Journal of Cellular Physiology·Mohamed-Kheir Idris AnasJay M Baltz
Sep 1, 1993·Molecular Reproduction and Development·R S PratherL J Van Winkle
Mar 1, 1993·Molecular Reproduction and Development·R S PratherL J Van Winkle
Mar 30, 2006·American Journal of Physiology. Endocrinology and Metabolism·Robyn L O'KaneRichard A Hawkins
Mar 17, 2005·The Journal of Pharmacology and Experimental Therapeutics·T Z SuM L Weber
Mar 1, 1995·The American Journal of Physiology·T C FureszC H Smith
Aug 1, 1991·The American Journal of Physiology·T C FureszC H Smith
Mar 26, 1998·Biochemical and Biophysical Research Communications·P ParentiB Giordana
Sep 30, 1991·Biochimica Et Biophysica Acta·L J Van WinkleA L Campione

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