PMID: 7543162Jul 1, 1995Paper

Inhibition of tumor cell metastasis in chick embryo by beta 1 integrin antisense oligonucleotide

Nihon rinsho. Japanese journal of clinical medicine
I NinomiyaT Sasaki

Abstract

The antimetastatic effect of integrin beta 1 subunit antisense oligonucleotide on human fibrosarcoma cell (HT-1080) in chick embryo was examined. Phosphorothioate modified antisense oligonucleotide (B1-1) was designed to hybridize specifically to integrin beta 1 subunit mRNA. Pretreatment of HT-1080 cells with B1-1 decreased the metastatic ability in chick embryonic liver and lung, while B1-1 showed no toxicity to HT-1080 nor chick embryo. Western blot analysis showed a reduction of the amount of cell surface integrin beta 1 subunit. B1-1 also inhibited HT-1080 attachment to laminin and fibronectin. These results indicate that the inhibition of tumor cell attachment by antisense oligonucleotide might be a good approach to the prevention of cancer metastasis.

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