Inhibition of Tumor Growth by Dietary Indole-3-Carbinol in a Prostate Cancer Xenograft Model May Be Associated with Disrupted Gut Microbial Interactions

Nutrients
Yanbei WuThomas T Y Wang

Abstract

Accumulated evidence suggests that the cruciferous vegetables-derived compound indole-3-carbinol (I3C) may protect against prostate cancer, but the precise mechanisms underlying its action remain unclear. This study aimed to verify the hypothesis that the beneficial effect of dietary I3C may be due to its modulatory effect on the gut microbiome of mice. Athymic nude mice (5⁻7 weeks old, male, Balb c/c nu/nu) with established tumor xenografts were fed a basal diet (AIN-93) with or without 1 µmoles I3C/g for 9 weeks. The effects of dietary I3C on gut microbial composition and microbial species interactions were then examined by 16s rRNA gene-based sequencing and co-occurrence network analysis. I3C supplementation significantly inhibited tumor growth (p < 0.0001) and altered the structure of gut microbiome. The abundance of the phylum Deferribacteres, more specifically, Mucispirillum schaedleri, was significantly increased by dietary I3C. Additionally, I3C consumption also changed gut microbial co-occurrence patterns. One of the network modules in the control group, consisting of seven bacteria in family S-27, was positively correlated with tumor size (p < 0.009). Moreover, dietary I3C disrupted microbial interactions and altered ...Continue Reading

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Citations

Mar 28, 2020·Nutrients·Luigi Mandrich, Emilia Caputo
Sep 8, 2019·Current Urology Reports·Karen M Wheeler, Michael A Liss
Jan 21, 2021·Cancers·Stefania De SantisMarcello Chieppa
Mar 27, 2021·Journal of Chromatography. a·Albatul Y AlmushaytiMichael J Scotter
Jul 29, 2021·Environmental Science and Pollution Research International·Amr El-SayedMohamed Kamel

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Methods Mentioned

BETA
xenograft
PCR
chip

Software Mentioned

Phylogenetic Molecular Ecological Network pipeline
PyNAST
QIIME pipeline
FastQC
PandaSeq
QIIME
Cytoscape
LEfSE
MiSeq Control Software ( MCS )

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