Inhibition of type 1 protein phosphatase activity by activation of beta-adrenoceptors in ventricular myocardium

Biochemical Pharmacology
Ramesh C GuptaH N Sabbah

Abstract

The regulation of protein phosphatase (PP) activity by cardiac beta-adrenergic receptor stimulation with isoproterenol (ISO) was studied in four groups of guinea pigs consisting of seven animals each. Group 1 received the vehicle solution only intraperitoneally; group 2, 6 microg/kg of ISO; group 3, 60 microg/kg of ISO; and group 4, 600 microg/kg of ISO. Total PP activity (consisting of both type 1 and type 2A PP), activity of each PP subtype, the cAMP-dependent protein kinase activity ratio (-cAMP/+cAMP), the phosphorylation of PP inhibitor 1, and the phosphorylation of phospholamban were measured in ventricular tissue. PP activity was also studied in ventricular cardiomyocytes isolated from guinea pigs treated with and without 1 microM ISO or 1 microM ISO plus 10 microM propranolol, an antagonist of the beta-adrenoceptor. PP activity decreased significantly in membrane vesicles, but not in cytosolic fractions, of guinea pigs treated with 60 and 600 microg/kg of ISO compared with untreated animals. The PKA activity ratio, PLB phosphorylation, and PP inhibitor 1 phosphorylation increased in ventricles of guinea pigs treated with 60 and 600 microg/kg of ISO compared with vehicle-treated animals. The decrease in overall PP activi...Continue Reading

References

Jan 1, 1988·Progress in Biophysics and Molecular Biology·H C Hartzell
Jan 1, 1988·Molecular and Cellular Endocrinology·R B IyerM A Kirchberger
Apr 1, 1995·Naunyn-Schmiedeberg's Archives of Pharmacology·N ZimmermannH Scholz
Jan 1, 1994·Advances in Enzyme Regulation·A A Depaoli-RoachS Zolnierowicz
Nov 1, 1993·Cardiovascular Research·S BartelE G Krause
Apr 1, 1956·Biochimica Et Biophysica Acta·E G KREBS, E H FISCHER
Jun 18, 2004·Annals of the New York Academy of Sciences·Marius P SumandeaR John Solaro

❮ Previous
Next ❯

Citations

Aug 24, 2007·Clinical and Experimental Pharmacology & Physiology·P MolenaarA J Kaumann
Aug 12, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Nicole BrüchertJoachim Neumann
Apr 28, 2004·Biochemical and Biophysical Research Communications·Ali El-ArmoucheThomas Eschenhagen
Mar 7, 2007·Circulation·Shao-kui WeiMark C P Haigney
Aug 9, 2006·Circulation·Ali El-ArmoucheDobromir Dobrev
May 24, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Ravi RajashreePolly A Hofmann
Mar 1, 2012·British Journal of Pharmacology·A Rietz, Jp Spiers
Jan 13, 2004·Physiological Reviews·Hugo Ceulemans, Mathieu Bollen
Nov 14, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Ramesh C GuptaHani N Sabbah
Feb 14, 2015·Clinical and Experimental Pharmacology & Physiology·Chanchal ChandramouliLea M D Delbridge

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.