Inhibition of tyrosine kinase receptor type B synthesis blocks axogenic effect of estradiol on rat hypothalamic neurones in vitro

The European Journal of Neuroscience
V BritoM J Cambiasso

Abstract

17-beta-estradiol (E2) increases axonal growth and tyrosine kinase receptor (Trk)B levels of male-derived hypothalamic neurones in vitro. To investigate whether the axogenic response depends on the upregulation of TrkB, we analysed neuritic growth and neuronal polarization in cultures treated with an antisense oligonucleotide against TrkB mRNA. In cultures without E2, treatment with 7.5 or 10 micro m antisense reduced TrkB levels and the percentage of neurones showing an identifiable axon; the number and length of minor processes were increased. In cultures treated with 5 micro m antisense, morphometric parameters were normal although total TrkB levels were reduced. The same dose prevented the E2-dependent increase of TrkB levels and suppressed the axogenic effect of E2. These results indicate that TrkB is necessary for normal neuronal growth and maturation and further suggest that an increase in TrkB is necessary for E2 to exert its axogenic effect in male-derived neurones.

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Citations

Jan 16, 2008·Physiological Reviews·Margaret M McCarthy
May 6, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Joanna L Spencer-SegalTeresa A Milner
Oct 6, 2011·The Journal of Steroid Biochemistry and Molecular Biology·María Angeles ArevaloLuis M Garcia-Segura
Dec 15, 2007·Frontiers in Neuroendocrinology·Joanna L SpencerBruce S McEwen
Feb 22, 2005·Current Opinion in Neurobiology·Richard B Simerly
Feb 11, 2015·Human Reproduction·Jocelyn M WesselsWarren G Foster
Apr 20, 2019·Frontiers in Cellular Neuroscience·Lucas E Cabrera ZapataMaría Julia Cambiasso

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