Inhibition of vancomycin-resistant enterococci by an in vitro continuous-flow competitive exclusion culture containing human stool flora

The Journal of Infectious Diseases
C J DonskeyL B Rice


An in vitro anaerobic continuous-flow competitive exclusion (CFCE) culture model was used to study the ability of human stool flora to inhibit the growth of vancomycin-resistant (VR) enterococci (VRE). The CFCE culture was established from a stool sample obtained from a healthy adult. When 10(3) or 10(6) cfu/mL of VR Enterococcus faecium were added to the CFCE culture, the VRE were eliminated within 6 or 9 days, respectively. When 10(7) cfu/mL of the CFCE culture was added to a continuous-flow culture that contained 10(7) cfu/mL of VRE, the density of VRE was reduced but not eliminated. These data support the hypothesis that the indigenous intestinal flora inhibit growth of VRE and suggest that CFCE cultures may be a useful means to study interactions between the indigenous flora and VRE.


Jun 5, 2003·Journal of Microbiological Methods·Curtis J DonskeyL B Rice
Jun 11, 2002·Annual Review of Nutrition·Lora V HooperJeffrey I Gordon
Jun 15, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E M Mascini, M J Bonten
Mar 8, 2008·International Journal of Medical Microbiology : IJMM·Wolfgang WitteGuido Werner
Jun 7, 2012·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·James A McKinnellLoren G Miller
Jun 16, 2017·Annual Review of Microbiology·Brittany B Lewis, Eric G Pamer

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