Inhibition of vascular smooth muscle cell growth by inhibition of fibronectin matrix assembly

Circulation Research
K O Mercurius, A O Morla

Abstract

The regulation of vascular smooth muscle cell (VSMC) proliferation by the fibronectin matrix was tested by treating human umbilical artery smooth muscle cells (HUASMCs) with a recombinant fragment of fibronectin (protein III1-C) that has previously been shown to modulate fibronectin matrix assembly. III1-C inhibited HUASMC proliferation by 75% to 90%. The inhibition of growth was time dependent; III1-C had no effect on DNA synthesis after 0 to 5 hours of treatment but did have an effect at 24 hours and beyond. III1-C did not stimulate apoptosis in these cells, indicating that the inhibition of proliferation was not due to an induction of programmed cell death. The effects of III1-C on cell growth were only specific for normal diploid smooth muscle cells. III1-C had no effect on the proliferation of IMR-90 fibroblasts, endothelial cells, NIH 3T3 cells, or the rat aortic smooth muscle cell line A7r5. However, III1-C did inhibit proliferation by primary rat aortic smooth muscle cells. An analysis of HUASMC fibronectin receptor (integrin alpha5beta1) distribution revealed that III1-C did not inhibit alpha5beta1 localization to focal contacts. Moreover, III1-C had no effect on the relative expression levels of seven different integr...Continue Reading

References

Jan 1, 1979·Physiological Reviews·J Chamley-CampbellR Ross
Jan 1, 1992·Cold Spring Harbor Symposia on Quantitative Biology·L H RomerC E Turner
Apr 1, 1992·The Journal of Cell Biology·S E LaFlammeK M Yamada
Nov 11, 1992·Journal of the American College of Cardiology·R S SchwartzE J Topol
Oct 1, 1992·Current Opinion in Cell Biology·D F MosherJ A McDonald
Jun 1, 1991·The Journal of Cell Biology·J E Schwarzbauer
Nov 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J ThybergB A Bottger
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P A MaherS J Singer
Jun 8, 1987·FEBS Letters·M A ChernousovV E Koteliansky
Jan 1, 1987·Differentiation; Research in Biological Diversity·U Hedin, J Thyberg
Apr 1, 1985·Experimental and Molecular Pathology·G R Campbell, J H Campbell
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M D Pierschbacher, E Ruoslahti
Jul 1, 1995·Physiological Reviews·G K Owens
Apr 14, 1995·Science·E A Clark, J S Brugge
Feb 1, 1994·The Journal of Cell Biology·S M Frisch, H Francis
Sep 1, 1993·Molecular Biology of the Cell·J E MeredithM A Schwartz
Jan 1, 1996·The Journal of Cell Biology·K WennerbergR Fässler
Oct 1, 1995·Current Opinion in Cell Biology·C D RoskelleyM J Bissell
Apr 1, 1996·The Journal of Cell Biology·D C HockingP J McKeown-Longo
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·M MrksichG M Whitesides
Dec 1, 1996·The Journal of Clinical Investigation·R K Assoian, E E Marcantonio
Jan 13, 1997·The Journal of Cell Biology·R K Assoian
Jan 1, 1997·Annual Review of Physiology·D E Ingber
May 30, 1997·Science·C S ChenD E Ingber

❮ Previous
Next ❯

Citations

Oct 26, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Philipp Stawowy, Eckart Fleck
Oct 27, 2004·Archives of Dermatological Research·Sompid KintarakSimon A Whawell
Feb 27, 2004·Biochimica Et Biophysica Acta·Jane Sottile
Oct 6, 1999·Current Opinion in Cell Biology·J E Schwarzbauer, J L Sechler
Apr 21, 1999·Current Opinion in Cell Biology·S M Schoenwaelder, K Burridge
May 7, 2003·Trends in Cardiovascular Medicine·Yeshao WenJerry Nadler
Jan 12, 2011·Nature Reviews. Neurology·Jose GutierrezClinton B Wright
Jan 14, 1999·The New England Journal of Medicine·R Ross
Nov 19, 2010·The Journal of Biological Chemistry·Craig T LefortDenise C Hocking
Mar 23, 2000·The Journal of Experimental Medicine·R K SuniaraJ J Owen
Oct 21, 2009·The Journal of Experimental Medicine·Per FogelstrandMark H Ginsberg
Nov 26, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Kaivan KhavandiAnthony M Heagerty
May 3, 2008·Genes & Development·Xiaoming ZhouStephen J Weiss
Oct 17, 2009·Journal of Biomechanical Engineering·Qingping YaoHai-Chao Han
May 2, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Hou-Yu ChiangJane Sottile
Dec 4, 2012·Stem Cell Research & Therapy·Min Zhang, Bing Huang
Dec 14, 2011·Journal of Cell Science·Feng Shi, Jane Sottile
Jun 29, 2000·Biochemical and Biophysical Research Communications·A O Morla, J E Mogford
Jan 12, 2011·The Journal of Cell Biology·Vinicio A de Jesus PerezMarlene Rabinovitch
Oct 8, 1998·The Journal of Cell Biology·S BourdoulousE Ruoslahti
Apr 25, 2006·Free Radical Biology & Medicine·Hong-Sheng Zhang, Sheng-Qi Wang
Feb 11, 2014·Matrix Biology : Journal of the International Society for Matrix Biology·Candace D GildnerDenise C Hocking
Jul 15, 2015·Surgical Neurology International·Yoshifumi HoritaNobuhiro Mikuni
Jan 7, 2009·Therapeutic Advances in Cardiovascular Disease·Kazuhiko SonoyamaTony Heagerty
Apr 1, 2000·The Journal of Biological Chemistry·D C HockingK J Langenbach
Sep 20, 2006·The Journal of Biological Chemistry·Liqiong GuiDenise C Hocking
Oct 12, 2007·American Journal of Physiology. Cell Physiology·Jane SottileJennifer Chandler
May 28, 2014·The Journal of Experimental Medicine·Claudio RaimondiChristiana Ruhrberg
Jun 13, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lida Guo, Chuanyue Wu
Mar 6, 1999·The Journal of Biological Chemistry·K J Langenbach, J Sottile
Apr 27, 2001·The Journal of Biological Chemistry·B R Tomasini-JohanssonD F Mosher
Sep 25, 1998·The Journal of Biological Chemistry·J L Sechler, J E Schwarzbauer
Feb 13, 2003·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Benoît LarivièreAndré D Beaulieu

❮ Previous
Next ❯

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