PMID: 20646593Jul 22, 2010Paper

Inhibitive effect and mechanism of monoclonal antibody targeted SEA on human bladder cancer cell line BIU-87

Zhonghua yi xue za zhi
Zhen GongWenhao Tang

Abstract

To investigate the in vitro inhibitive effect and apoptotic mechanism of BDI-1 monoclonal antibody targeted SEA on human bladder cancer cell line BIU-87. Human peripheral blood mononuclear cells (PBMC) and human bladder cancer cell line BIU-87 were used as effector and target cells respectively in three experimental groups, control group, SEA group and monoclonal antibody targeted SEA group, treated with different concentrations of SEA and targeted SEA respectively in the latter two. The inhibition rates of target cells were measured by cell counting kit-8 (CCK-8) assay. Apoptosis was determined by fluorescent Hocehst 33258 staining and flow cytometry (FCM). The expression of Bax, Bcl-2 proteins and cytokine concentration of co-culture supernatants were detected by Western blot and ELISA respectively. Compared with those of control group (from 5.8% +/- 3.0% to 16.5% +/- 4.0%) and SEA group (from 20.7% +/- 2.1% to 68.6% +/- 4.0%), the inhibition rates in targeted SEA group increased significantly (from 31.2% +/- 2.6% to 88.7% +/- 3.0%, P < 0.05). Characteristic changes of apoptosis were observed in both experimental treatment groups, especially in targeted SEA group. FCM also showed more prominent apoptosis induced in targeted S...Continue Reading

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis