Inhibitive Effect of Eugenol and Its Nanoemulsion on Quorum Sensing-Mediated Virulence Factors and Biofilm Formation by Pseudomonas aeruginosa

Journal of Food Protection
Z LouRebaone Letsididi

Abstract

The aim of the present study was to evaluate the quorum sensing (QS) inhibition potential of eugenol and eugenol nanoemulsion against QS-dependent virulence factor production and gene expression, as well as biofilm formation in Pseudomonas aeruginosa. In the current study, eugenol nanoemulsion at a sub-MIC of 0.2 mg/mL specifically inhibited about 50% of the QS-mediated violacein production in Chromobacterium violaceum, as well as the production of N-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-C12-HSL) and C4-HSL N-acyl homoserine lactone signal molecules, pyocyanin, and swarming motility in P. aeruginosa. The inhibitive effect of eugenol and its nanoemulsion on the expression of the QS synthase genes was concentration dependent, displaying 65 and 52% expression level for lasI, respectively, and 61 and 45% expression level for rhlI, respectively, at a concentration of 0.2 mg/mL. In addition, the inhibitive effect of eugenol and its nanoemulsion on the expression of the rhlA gene responsible for the production of rhamnolipid was also concentration dependent, displaying 65 and 51% expression level for the rhlA gene, respectively, at a concentration of 0.2 mg/mL. Eugenol and its nanoemulsion also displayed 36 and 63% respective ...Continue Reading

References

May 19, 2006·Letters in Applied Microbiology·J H ChooJ-K Hwang
Mar 21, 2007·Applied and Environmental Microbiology·Takenori IshidaJunichi Kato
May 15, 2007·Fitoterapia·D A VattemR J C McLean
Aug 19, 2007·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Srdjan StepanovićFilip Ruzicka
Jan 24, 2008·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Thomas BjarnsholtMichael Givskov
Oct 29, 2008·Medicinal Research Reviews·Nanting NiBinghe Wang
Aug 19, 2009·Annual Review of Genetics·Wai-Leung Ng, Bonnie L Bassler
Oct 23, 2009·FEMS Immunology and Medical Microbiology·Ramalingam ThenmozhiShunmugiah Karutha Pandian
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mitchell T ButlerRasika M Harshey
Mar 15, 2011·Archives of Oral Biology·Sang-Eun MoonJeong-Dan Cha
Jan 1, 2010·Biotechnology & Genetic Engineering Reviews·Devendra H DusanePattanathu K S M Rahman
Jan 10, 2012·Archives of Medical Research·Sybiya Vasantha Packiavathy Issac AbrahamVeera Ravi Arumugam
Apr 27, 2012·Journal of Agricultural and Food Chemistry·Pilar TruchadoAna Allende
Sep 8, 2012·Ultrasonics Sonochemistry·Vijayalakshmi GhoshNatarajan Chandrasekaran
Sep 19, 2012·Journal of Computer-aided Molecular Design·Angusamy AnnapooraniArumugam Veera Ravi
Sep 4, 2013·Journal of Agricultural and Food Chemistry·Yuhua ChangDavid Julian McClements
Sep 24, 2013·Organic & Biomolecular Chemistry·Nira RabinMichael M Meijler
Oct 18, 2013·Indian Journal of Critical Care Medicine : Peer-reviewed, Official Publication of Indian Society of Critical Care Medicine·Jeetendra GurungPrithwis Bhattacharyya
Nov 5, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Nimmy AugustineSabu Thomas
Nov 12, 2013·World Journal of Microbiology & Biotechnology·Sheeba JayaseelanSelvakumar Dharmaraj
Nov 21, 2013·Colloids and Surfaces. B, Biointerfaces·Vijayalakshmi GhoshNatarajan Chandrasekaran
Nov 23, 2013·Food Chemistry·Issac Abraham Sybiya Vasantha PackiavathyArumugam Veera Ravi
Apr 5, 2014·Microbiology and Immunology·Halkare Suryanarayana VasaviPunchapady-Devasya Rekha
Jun 30, 2014·Applied Microbiology and Biotechnology·Maisem LaabeiA Tobias A Jenkins
Dec 17, 2014·International Journal of Food Microbiology·Yong-Guy KimJintae Lee
Oct 23, 2015·International Journal of Food Microbiology·Hyeree Moon, Min Suk Rhee

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Citations

Nov 14, 2019·International Journal of Molecular Sciences·Dmitry DeryabinGalimjan Duskaev
Nov 27, 2019·Applied Microbiology and Biotechnology·Fazlurrahman KhanYoung-Mog Kim
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hamzah Basil MohammedAlina-Maria Holban
Jun 26, 2021·Frontiers in Microbiology·Jingyuan FuHualin Fu
Oct 27, 2021·Advanced Drug Delivery Reviews·Sajid AsgharThierry F Vandamme

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