PMID: 9653134Jul 8, 1998Paper

Inhibitor binding changes domain mobility in the iron-sulfur protein of the mitochondrial bc1 complex from bovine heart

Proceedings of the National Academy of Sciences of the United States of America
H KimJ Deisenhofer

Abstract

We have analyzed crystal structures of cytochrome bc1 complexes with electron transfer inhibitors bound to the ubiquinone binding pockets Qi and/or Qo in the cytochrome b subunit. The presence or absence of the Qi inhibitor antimycin A did not affect the binding of the Qo inhibitors. Different subtypes of Qo inhibitors had dramatically different effects on the mobility of the extramembrane domain of the iron-sulfur protein (ISP): Binding of 5-undecyl-6-hydroxy-4, 7-dioxobenzothiazol and stigmatellin (subtype Qo-II and Qo-III, respectively) led to a fixation of the ISP domain on the surface of cytochrome b, whereas binding of myxothiazol and methoxyacrylate-stilbene (subtype Qo-I) favored release of this domain. The native structure has an empty Qo pocket and is intermediate between these extremes. On the basis of these observations we propose a model of quinone oxidation in the bc1 complex, which incorporates fixed and loose states of the ISP as features important for electron transfer and, possibly, also proton transport.

References

Oct 21, 1976·Journal of Theoretical Biology·P Mitchell
Jan 1, 1986·Methods in Enzymology·G von Jagow, T A Link
Jan 1, 1985·Annual Review of Biochemistry·Y Hatefi
Jan 1, 1994·Critical Reviews in Biochemistry and Molecular Biology·U Brandt, B Trumpower
Jul 18, 1996·Biochimica Et Biophysica Acta·C A YuJ Deisenhofer
May 16, 1998·Nature·Z ZhangS H Kim
Jan 1, 1996·Acta Crystallographica. Section D, Biological Crystallography·K D Cowtan, P Main
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯

Citations

May 22, 2001·Archives of Biochemistry and Biophysics·L Gille, H Nohl
Dec 21, 2006·Folia Microbiologica·V Bethal
May 23, 2013·Photosynthesis Research·Alexander N Tikhonov
Apr 9, 2005·Trends in Biochemical Sciences·Artur OsyczkaP Leslie Dutton
Jul 17, 1999·Biochimica Et Biophysica Acta·R E Sharp, S K Chapman
Sep 7, 2000·Current Opinion in Biotechnology·A W MunroM D Walkinshaw
Jul 29, 2000·Bioorganic & Medicinal Chemistry Letters·C K RyuB H Lee
Jul 7, 2001·Trends in Biochemical Sciences·E DarrouzetF Daldal
Aug 21, 2003·Bioorganic & Medicinal Chemistry·Chung Kyu RyuMi Jin Chae
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yasuhiro Kashino
Nov 26, 2009·Journal of the American Chemical Society·Pei-Liang ZhaoGuang-Fu Yang
Dec 2, 2004·Nature Reviews. Molecular Cell Biology·Nathan Nelson, Adam Ben-Shem
Nov 28, 2001·European Journal of Biochemistry·R Covián, R Moreno-Sánchez
Apr 28, 2000·European Journal of Biochemistry·K KuwabaraH Miyoshi
Jan 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Xiaoyu LinYu Shen
Jan 16, 2010·The Journal of Biological Chemistry·Qiyu WangChang-An Yu
May 27, 2005·The Journal of Biological Chemistry·Michael W MatherAkhil B Vaidya
Aug 15, 2008·The Journal of Biological Chemistry·Buddha GurungChang-An Yu
Jun 8, 2006·Annual Review of Biochemistry·William A CramerJanet L Smith
Feb 24, 2004·Annual Review of Physiology·Antony R Crofts
Jul 4, 2006·Respiratory Research·Michael GrothRainer V Haberberger
Jan 8, 2009·Molecular Pain·Yi-Tin YenChih-Cheng Chen
Dec 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jiusheng YanWilliam A Cramer
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jian ZhuChang-An Yu
Jul 11, 2001·Journal of Molecular and Cellular Cardiology·E J LesnefskyC L Hoppel
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·E DarrouzetF Daldal
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·F CutruzzolaM Brunori
May 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jonathan L CapeDavid M Kramer
Aug 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lothar EsserDi Xia
Apr 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Huamin ZhangWilliam A Cramer
Feb 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·M BrugnaI Sinning
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·F SchulerJ E Casida
Feb 4, 2014·Plant Physiology and Biochemistry : PPB·Alexander N Tikhonov
Oct 8, 2011·Journal of Molecular Biology·S Saif HasanWilliam A Cramer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.