Continual spermatogenesis at a quantitatively normal level is required to sustain male fertility. The foundation of this process relies on maintenance of an undifferentiated spermatogonial population consisting of spermatogonial stem cells (SSCs) that self-renew as well as transient amplifying progenitors produced by differentiation. In mammals, type A(single) spermatogonia form the SSC population, but molecular markers distinguishing these from differentiating progenitors are undefined and knowledge of mechanisms regulating their functions is limited. We show that in the mouse male germline the transcriptional repressor ID4 is expressed by a subpopulation of undifferentiated spermatogonia and selectively marks A(single) spermatogonia. In addition, we found that ID4 expression is up-regulated in isolated SSC-enriched fractions by stimulation from GDNF, a key growth factor driving self-renewal. In mice lacking ID4 expression, quantitatively normal spermatogenesis was found to be impaired due to progressive loss of the undifferentiated spermatogonial population during adulthood. Moreover, reduction of ID4 expression by small interfering RNA treatment abolished the ability of wild-type SSCs to expand in vitro during long-term cult...Continue Reading
Overexpression of Id protein inhibits the muscle differentiation program: in vivo association of Id with E2A proteins
The spermatogonial stem cell population in adult rats. I. Their morphology, proliferation and maturation
The expression pattern of Id4, a novel dominant negative helix-loop-helix protein, is distinct from Id1, Id2 and Id3
Lymphoid-specific expression of the Id3 gene in hematopoietic cells. Selective antagonism of E2A basic helix-loop-helix protein associated with Id3-induced differentiation of erythroleukemia cells.
Spermatogonial stem cells share some, but not all, phenotypic and functional characteristics with other stem cells
Culture conditions and single growth factors affect fate determination of mouse spermatogonial stem cells
The first round of mouse spermatogenesis is a distinctive program that lacks the self-renewing spermatogonia stage
Functional identification of the actual and potential stem cell compartments in mouse spermatogenesis
Glial cell line-derived neurotrophic factor regulation of genes essential for self-renewal of mouse spermatogonial stem cells is dependent on Src family kinase signaling
Colony stimulating factor 1 is an extrinsic stimulator of mouse spermatogonial stem cell self-renewal
Identification of spermatogonial stem cell subsets by morphological analysis and prospective isolation
The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3
NEUROG3 is a critical downstream effector for STAT3-regulated differentiation of mammalian stem and progenitor spermatogonia
Id4 deficiency attenuates prostate development and promotes PIN-like lesions by regulating androgen receptor activity and expression of NKX3.1 and PTEN
Single cell analysis facilitates staging of Blimp1-dependent primordial germ cells derived from mouse embryonic stem cells
Characterization of spermatogonial stem cells lacking intercellular bridges and genetic replacement of a mutation in spermatogonial stem cells
Targeting the Gdnf Gene in peritubular myoid cells disrupts undifferentiated spermatogonial cell development
Quantitative analysis of male germline stem cell differentiation reveals a role for the p53-mTORC1 pathway in spermatogonial maintenance
Spermatogonial stem cells specific marker identification in channel catfish, Ictalurus punctatus and blue catfish, I. furcatus
Developments in techniques for the isolation, enrichment, main culture conditions and identification of spermatogonial stem cells
Evaluation of candidate spermatogonial markers ID4 and GPR125 in testes of adult human cadaveric organ donors
Phenotypic characterization and in vitro propagation and transplantation of the Nile tilapia (Oreochromis niloticus) spermatogonial stem cells
Fluorescence- and magnetic-activated cell sorting strategies to isolate and enrich human spermatogonial stem cells
Regulation of spermatogonial stem cell self-renewal and spermatocyte meiosis by Sertoli cell signaling
Endogenous, very small embryonic-like stem cells: critical review, therapeutic potential and a look ahead
Construction and analysis of a protein-protein interaction network related to self-renewal of mouse spermatogonial stem cells
DMRTC2, PAX7, BRACHYURY/T and TERT Are Implicated in Male Germ Cell Development Following Curative Hormone Treatment for Cryptorchidism-Induced Infertility
Cep55 overexpression causes male-specific sterility in mice by suppressing Foxo1 nuclear retention through sustained activation of PI3K/Akt signaling
Roles for Kisspeptin in proliferation and differentiation of spermatogonial cells isolated from mice offspring when the cells are cocultured with somatic cells
Optimization of Serum-Free Culture Conditions for Propagation of Putative Buffalo (Bubalus bubalis) Spermatogonial Stem Cells
Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes
Differential RA responsiveness directs formation of functionally distinct spermatogonial populations at the initiation of spermatogenesis in the mouse
A-single spermatogonia heterogeneity and cell cycles synchronize with rat seminiferous epithelium stages VIII-IX
Cped1 promotes chicken SSCs formation with the aid of histone acetylation and transcription factor Sox2
Differentiation of neonate mouse spermatogonial stem cells on three-dimensional agar/polyvinyl alcohol nanofiber scaffold.
Stage-specific embryonic antigen 4 is a membrane marker for enrichment of porcine spermatogonial stem cells
Mammalian SWI/SNF collaborates with a polycomb-associated protein to regulate male germline transcription in the mouse
Single cell RNA-sequencing identified Dec2 as a suppressive factor for spermatogonial differentiation by inhibiting Sohlh1 expression
Developmental kinetics and transcriptome dynamics of stem cell specification in the spermatogenic lineage
Sertoli cell-only phenotype and scRNA-seq define PRAMEF12 as a factor essential for spermatogenesis in mice
Maintenance of potential spermatogonial stem cells in vitro by GDNF treatment in a chondrichthyan model (Scyliorhinus canicula L.)
Responses to glial cell line-derived neurotrophic factor change in mice as spermatogonial stem cells form progenitor spermatogonia which replicate and give rise to more differentiated progeny
Roles for osteocalcin in proliferation and differentiation of spermatogonial cells cocultured with somatic cells
Mechanisms regulating mammalian spermatogenesis and fertility recovery following germ cell depletion
Epigenetic Regulation of Spermatogonial Stem Cell Homeostasis: From DNA Methylation to Histone Modification
Retinoblastoma protein (RB1) controls fate determination in stem cells and progenitors of the mouse male germline
Effect of a Freezing Medium Containing Melatonin on Markers of Pre-meiotic and Post-meiotic Spermatogonial Stem Cells (SSCs) After Transplantation in an Azoospermia Mouse Model Due to Testicular Torsion.
Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine.
Spermatogonial Gene Networks Selectively Couple to Glutathione and Pentose Phosphate Metabolism but Not Cysteine Biosynthesis.
Transcriptome analysis revealed differences in the microenvironment of spermatogonial stem cells in seminiferous tubules between pre-pubertal and adult buffaloes.
Pentoxifylline improves the survival of spermatogenic cells via oxidative stress suppression and upregulation of PI3K/AKT pathway in mouse model of testicular torsion-detorsion.
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