Inhibitors of Ca2+ ATPase pump of sarcoplasmic reticulum attenuate reperfusion stunning in isolated rat heart

Journal of Cardiovascular Pharmacology
E F du Toit, L H Opie

Abstract

We tested for the first time the hypothesis that lessened uptake of Ca2+ into sarcoplasmic reticulum by inhibitors of the Ca(2+)-ATPase pump can decrease the severity of reperfusion stunning (postischemic mechanical dysfunction). We used two novel inhibitors of the Ca(2+)-ATPase pump: (a) cyclopiazonic acid (CPA, 10(-6)-10(-8)M), and (b) thapsigargin (10(-6) or 2.5 x 10(-8)M). The isolated working rat heart was subjected to 20-min global ischemia before 20-min reperfusion. The inhibitor was added either before onset of ischemia or at time of reperfusion. Reperfusion mechanical function (aortic output, AO) was measured and compared with the preischemia values. Pretreatment with CPA improved recovery of AO after 20-min reperfusion from 78.2 +/- 3.0 (n = 12) to 93.3 +/- 1.6% (n = 7) (p < 0.002) while CPA added during reperfusion only, improved AO recovery from 78.2 +/- 3.0 (n = 12) to 90.2 +/- 2.7% (n = 6) (p < 0.05). Pretreatment with thapsigargin (2.5 x 10(-8) M) improved reperfusion AO recovery from 63.5 +/- 1.1 (n = 6) to 96.8 +/- 4.2% (n = 6) (p < 0.002), but when thapsigargin was added only during reperfusion AO recovery did not change. We conclude that inhibition of the Ca2+ uptake pump represents a new principle of control...Continue Reading

Citations

Jun 10, 1998·The Japanese Journal of Thoracic and Cardiovascular Surgery : Official Publication of the Japanese Association for Thoracic Surgery = Nihon Kyōbu Geka Gakkai Zasshi·Y KumadaH Hirose
Apr 24, 2001·Pharmacology & Therapeutics·R ZucchiS Ronca-Testoni
Nov 20, 1998·Journal of Cardiovascular Pharmacology·M AvellanalS Barrigon
Dec 25, 2002·Journal of Cardiovascular Pharmacology·Patricia RodriguezSantos Barrigon
Apr 9, 2008·Physiological Reviews·Elizabeth Murphy, Charles Steenbergen
Jul 1, 1995·Basic Research in Cardiology·L H Opie
Jul 17, 2004·Free Radical Biology & Medicine·Mohammad Nouri SharikabadOdd Brørs
Nov 21, 2014·Pflügers Archiv : European journal of physiology·Hyoung Kyu KimJin Han
Nov 4, 2009·Journal of Molecular and Cellular Cardiology·Young-Hwan SongWon-Kyung Ho
Apr 9, 2009·Progress in Biophysics and Molecular Biology·Nina S GhaisChristopher L-H Huang
Jan 22, 2008·American Journal of Physiology. Heart and Circulatory Physiology·M A Hassan TalukderJay L Zweier
Aug 30, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Jarvis ChenElizabeth Murphy
Jul 17, 2007·American Journal of Physiology. Heart and Circulatory Physiology·M A Hassan TalukderJay L Zweier
Aug 2, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Ken YamamuraElizabeth Murphy

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