Abstract
We assessed the possible role of interleukin-1beta-converting enzyme-family proteases (caspases) in apoptosis in cultured rat cerebellar granule neurons. CPP32 (caspase-3)-like protease activity was augmented by low KCl treatment, preceding neuronal cell death. Agents such as brain-derived neurotrophic factor (BDNF), dibutylyl cAMP, NMDA, actinomycin D, S-adenosyl-L-methionine, and spermine prevented apoptosis. For various neuroprotective agents, the degree of apoptosis prevention correlated with the prevention of the activation of CPP32-like protease. Furthermore, Z-Asp-2, 6-dichlorobenzoyloxy-methylketone (Z-Asp-CH2-DCB), Boc-Asp-fluoromethylketone (Boc-Asp-FMK), and Z-Val-Ala-Asp-fluoromethylketone (Z-VAD-FMK), which are inhibitors of caspases, also prevented apoptosis. In contrast to many other neuroprotective agents, these inhibitors of caspases showed little effect on the decrease of cellular 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) reduction activity after low KCl treatment. The neurons rescued by these inhibitors of caspases during low KCl treatment were in a hypoenergic state in their ATP levels and vulnerable to subsequent treatment with medium containing high KCl or glutamate which induce a...Continue Reading
References
Sep 13, 1991·Journal of Immunological Methods·N W RoehmA L Glasebrook
Nov 19, 1990·Brain Research·J Y KohC W Cotman
Jun 7, 1988·Brain Research·A NovelliR C Henneberry
Nov 1, 1988·Neuroscience·R BalázsN Hack
Apr 18, 1995·Brain Research. Developmental Brain Research·T KuboH Hatanaka
Oct 5, 1995·Nature·A J DarmonR C Bleackley
Dec 1, 1995·Journal of Neurochemistry·I KanekoS Tutumi
Jul 6, 1995·Nature·D W NicholsonY A Lazebnik
May 1, 1995·Trends in Biochemical Sciences·S Kumar
Apr 26, 1995·Biochemical and Biophysical Research Communications·T MashimaT Tsuruo
Jun 2, 1995·Cell·M TewariV M Dixit
Jul 1, 1995·Journal of Neurochemistry·M S ShearmanV J Tailor
Nov 19, 1993·Cell·M MiuraJ Yuan
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S R D'MelloP Calissano
Feb 11, 1994·Science·V GagliardiniJ Yuan
Jun 1, 1993·Archives of Biochemistry and Biophysics·M V Berridge, A S Tan
Jan 26, 1996·The Journal of Biological Chemistry·J SchlegelD W Nicholson
Apr 25, 1996·Nature·M EnariS Nagata
Feb 15, 1996·European Journal of Biochemistry·A J HaleG T Williams
May 1, 1996·Genes & Development·D XueH R Horvitz
Jul 12, 1996·The Journal of Biological Chemistry·R C ArmstrongK J Tomaselli
Oct 18, 1996·Cell·E S AlnemriJ Yuan
Aug 12, 1996·Neuroreport·A J PatelH A Rohan de Silva
Nov 28, 1996·Nature·K KuidaR A Flavell
Dec 1, 1996·The Journal of Cell Biology·M DeshmukhE M Johnson
Dec 1, 1996·Trends in Neurosciences·L M Schwartz, C E Milligan
Dec 20, 1996·Neuroreport·R NathK K Wang
Mar 14, 1997·The Journal of Biological Chemistry·A J Polverino, S D Patterson
Apr 11, 1997·Brain Research·J Harada, M Sugimoto
Aug 1, 1997·Journal of Neurochemistry·Y LiuD Schubert
Nov 8, 1963·Biochimica Et Biophysica Acta·T F SLATERU STRAEULI
Citations
Aug 15, 2000·European Journal of Pharmacology·G L WenkS Fiorucci
Mar 18, 2000·Journal of Neuroscience Methods·D Lobner
Apr 12, 2003·Neurochemistry International·Yun-Chia ChouChiao Mei Lin
May 7, 2003·Neuroscience·K A Galvin, D E Oorschot
Jan 13, 2000·Neuropathology and Applied Neurobiology·J P SteinbachA Aguzzi
Feb 8, 2005·American Journal of Respiratory Cell and Molecular Biology·Stefanie KrickFrank Rose
May 7, 1999·Japanese Journal of Pharmacology·J Harada, M Sugimoto
Mar 10, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Lanhai LüJie Xu
Sep 8, 2007·Molecular and Cellular Neurosciences·Pu-Ting XuJohn R Gilbert
May 25, 2005·British Journal of Pharmacology·Ester VerdaguerMercè Pallàs
Jan 5, 2000·Physiological Reviews·D G Nicholls, S L Budd
Apr 12, 2000·Journal of Neuropathology and Experimental Neurology·L A SelznickK A Roth
Jul 31, 1999·Journal of Neurochemistry·J MoranD Pleasure
Jul 31, 1999·Journal of Neurochemistry·P J KinghamJ M Pocock
Feb 24, 2001·Developmental Biology·K L Hoffman, J C Weeks