Inhibitors of N-linked oligosaccharide processing glucosidases interfere with oligodendrocyte differentiation in culture

Journal of Neuroscience Research
Narayan R Bhat, P Zhang

Abstract

Previous studies have demonstrated that inhibitors of glycoprotein processing glucosidases interfere with the development of oligodendrocyte properties in primary cultures of embryonic rat brain cells (Bhat, J Neurosci Res 20:158-164, 1988). The present study examines the effect of castanospermine, an inhibitor of the processing glucosidases, on the development and differentiation of isolated oligodendrocyte progenitor cells. Treatment of oligodendrocyte progenitors with castanospermine did not affect the developmental progression of the precursors to become committed oligodendrocytes as revealed by comparable increases in the percentages of cells positive for galactocerebroside (a surface marker for terminally differentiated oligodendrocytes) in control and drug-treated cultures. On the other hand, there was an impairment of the expression of differentiated properties of oligodendrocytes [i.e., sulfolipid synthesis, myelin basic protein (MBP)] and 2'3'-cyclic nucleotide 3'-phosphohydrolase in the drug-treated cultures. Immunocytochemical analysis with anti-MBP antibodies revealed a reduced number of MBP-positive cells in inhibitor-treated cultures. Furthermore, a majority of MBP-positive cells in such cultures displayed immuno...Continue Reading

References

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Citations

Sep 13, 2003·Journal of Neurochemistry·Narayan R BhatFan Fan
Aug 19, 2014·Journal of Neuroscience Research·Antje AnjiMeena Kumari
Jan 20, 2007·Neurochemical Research·Narayan R BhatSangeeta B Mohanty

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