Inhibitors of poly(ADP-ribose) polymerase block nitric oxide-induced apoptosis but not differentiation in human leukemia HL-60 cells

Biochemical and Biophysical Research Communications
M L KuoS G Shiah

Abstract

Previous studies have shown that chemically generated nitric oxide (NO) can induce human leukemia HL-60 cells to undergo monocytic differentiation. We show here that exposure of HL-60 cells to chemical NO generators induces cell death via apoptosis which was examined by morphological and biochemical criteria. The activation of poly(ADP-ribose) polymerase (pADPRp) was found to occur during the process of cell death. The NO-induced apoptosis of HL-60 cells was effectively prevented by the ADP-ribosylation inhibitors nicotinamide (NA) and 3-aminobenzamide (3-AB). Since NO not only induced apoptosis but also triggered differentiation under the same concentration, we thus examined whether pADPRp participated in monocytic differentiation. It is of interest to note that the NO-mediated monocytic differentiation was not affected by 3-AB or NA. These findings indicate that activation of pADPRp is specifically involved in NO-induced apoptosis but not differentiation.

Citations

Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Paul O HassaMichael O Hottiger
Feb 11, 1999·Bioscience, Biotechnology, and Biochemistry·S OgataH Taguchi
Mar 1, 1997·Free Radical Research·M YabukiK Utsumi
Mar 12, 2002·The Journal of Investigative Dermatology·D S RosenthalM E Smulson
Jan 27, 1998·Nitric Oxide : Biology and Chemistry·C Szabó, H Ohshima
Apr 30, 2003·Cell Death and Differentiation·E L TaylorA G Rossi
Jul 25, 2008·American Journal of Physiology. Cell Physiology·Hsiu-Ni KungKuo-Shyan Lu
Sep 18, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·T TokimeK Ueda
Jul 31, 1998·European Journal of Pharmacology·C Szabó
Dec 31, 1998·Free Radical Biology & Medicine·L VirágC Szabó
Aug 15, 1998·Trends in Pharmacological Sciences·C Szabó, V L Dawson
Jan 1, 1997·Clinical Oncology : a Journal of the Royal College of Radiologists·G M Tozer, S A Everett
Feb 1, 2005·European Journal of Cell Biology·Hichem GallalaKonrad Sandhoff

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis