Inhibitors of protein phosphatase-2A: topography and subcellular localization

Brain Research. Molecular Brain Research
Hitoshi TanimukaiK Iqbal

Abstract

The mRNA and protein expressions of I1(PP2A) and I2(PP2A), the two inhibitors of protein phosphatase 2A (PP2A) were investigated in adult rat brain. The rat brain and human brain inhibitors showed similar molecular weights by Western blots. The cDNA probes for human brain I1(PP2A) and I2(PP2A) readily hybridized with the corresponding mRNAs of rat brain inhibitors in Northern blots. We detected 3.7 and 2.1 kb transcripts of I1(PP2A) and 2.9 and 2.0 kb transcripts of I2(PP2A) in rat brain. In situ hybridization revealed that the mRNAs of the two inhibitors were mainly localized in neurons. Strong expression of both I1(PP2A) and I2(PP2A) mRNAs were observed in the olfactory bulb, hippocampal pyramidal and dentate granule cell layers, and cerebellar Purkinje cell, granular and molecular layers. Moderate expression of I1(PP2A) and I2(PP2A) mRNAs were observed in the cerebral cortex, caudate putamen, thalamus, hypothalamus, amygdala and pontine nucleus. The expression of I1(PP2A) and I2(PP2A) and as well as of PP-2A was also investigated by immunohistochemistry using antibodies to each protein. The distribution patterns of the two inhibitor proteins were similar to those of their corresponding mRNAs and to the expression of PP-2A. W...Continue Reading

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Citations

Aug 13, 2011·The Journal of Biological Chemistry·Shaojuan LaiHarry T Orr
Feb 5, 2008·The Journal of Biological Chemistry·She ChenKhalid Iqbal
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Jul 17, 2014·The Journal of Biological Chemistry·Jimcy PlatholiHugh C Hemmings
May 16, 2018·Frontiers in Molecular Neuroscience·Qing ZhangXiaochuan Wang

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