Inhibitors of RNA synthesis and passage of chick embryo fibroblasts through the G1 period

Journal of Cellular Physiology
D E ChadwickI Lieberman

Abstract

Events that are essential for progression through the G1 period begin immediately or shortly after resting chick embryo cells are given fresh medium with serum. The following observations support the contention that the critical events include the production of non-ribosomal RNAs: (1) Addition to the "shift-up" medium of either of two inhibitors of RNA formation, camptothecin or 5, 6-dichloro--1-beta-D-ribofuranosylbenzimidazole, delays the onset of DNA replication by about the length of time the cells are exposed to the drugs. (2) Although entry into the S phase is delayed by the inhibitors, the slopes of the DNA response curves are identical to that of control cultures. (3) Neither drug reduces significantly the rate of overall protein synthesis. Observations (2) and (3) are taken to mean that expansion of the G1 period is not due to cell damage. (4) A third inhibitor of RNA synthesis, cordycepin, also delays passage of stimulated cells through the G1 phase, but, in this case, the length of the delay period is greater than that of the exposure period. (5) A low dose of actinomycin D does not impede movement towards the S phase, even though the synthesis of preribosomal RNA is considerably reduced. The possibility is considere...Continue Reading

References

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Citations

Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·I TammJ S Murphy
Jun 15, 1981·International Journal of Cancer. Journal International Du Cancer·D Lundblad, E Lundgren
Sep 1, 1984·Molecular and Cellular Biology·J Campisi, A B Pardee

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