Inhibitors of the polymerase chain reaction in Papanicolaou stain. Removal with a simple destaining procedure

Acta Cytologica
J T ChenD P Clark

Abstract

To identify inhibitors of the polymerase chain reaction (PCR) within Papanicolaou stain and to develop a method of removal. PCR was performed on cellular digests of archival, Papanicolaou-stained cervical smears and unstained cervical smears using primers to the human beta-globin gene to determine if Papanicolaou stain inhibited the reaction. Next, in order to identify the specific reagents responsible for inhibition, components of the Papanicolaou stain were added to PCRs performed on purified human genomic DNA. Finally, PCR was performed on cellular digests of cervical smears that had been destained using 1% HCl to determine if the inhibitors of PCR were removed. Cellular digests from Papanicolaou-stained cervical smears did not yield products from PCR, whereas cellular digests from unstained cervical smears always yielded PCR products. Analysis of individual Papanicolaou stain reagents revealed inhibition of PCR by hematoxylin and aluminum sulfate. These inhibitors could be removed from Papanicolaou-stained cervical smears by destaining the slides with 1% HCl. The hematoxylin and aluminum sulfate within Papanicolaou stain inhibit PCR but can be removed from Papanicolaou-stained cytologic specimens using a simple destaining p...Continue Reading

Citations

Feb 1, 2005·The Journal of Molecular Diagnostics : JMD·Kathleen M MurphyJames R Eshleman
Nov 26, 2002·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·M E CruickshankH C Kitchener
Feb 12, 1998·Thyroid : Official Journal of the American Thyroid Association·Y TakiyamaM A Zeiger
Jun 1, 2001·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·M T SiddiquiM Saji
Jun 8, 2001·American Journal of Clinical Pathology·B AvidorM Giladi
Oct 1, 2004·Cytopathology : Official Journal of the British Society for Clinical Cytology·K CanfellV Beral
Feb 7, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Deepa J BhandareVictor V Levenson Chernokhvostov
Jun 23, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Hironori TakagiMasao Hirose
Mar 4, 2000·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·T MuraseT Eimoto
Jun 13, 2000·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Y ManF A Tavassoli
Nov 8, 2002·Cytopathology : Official Journal of the British Society for Clinical Cytology·Amina E L HamidiMing-Qing Du
May 7, 2020·Histochemistry and Cell Biology·Alexandra PoteAna Marques-Ramos
Jun 14, 2000·Plastic and Reconstructive Surgery·I G Kara, F Fişekçi
Mar 22, 2014·Molecular and Clinical Oncology·Paola UliviDino Amadori

Related Concepts

Aluminum hydrogen sulfate
Alum Compounds
DNA, Double-Stranded
Stains
Globin
Haematoxylon
Staining and Labeling
Pap Smear
Nested Polymerase Chain Reaction
Papanicolaou Test

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