Inhibitors of the polymerase chain reaction in Papanicolaou stain. Removal with a simple destaining procedure

Acta Cytologica
J T ChenD P Clark


To identify inhibitors of the polymerase chain reaction (PCR) within Papanicolaou stain and to develop a method of removal. PCR was performed on cellular digests of archival, Papanicolaou-stained cervical smears and unstained cervical smears using primers to the human beta-globin gene to determine if Papanicolaou stain inhibited the reaction. Next, in order to identify the specific reagents responsible for inhibition, components of the Papanicolaou stain were added to PCRs performed on purified human genomic DNA. Finally, PCR was performed on cellular digests of cervical smears that had been destained using 1% HCl to determine if the inhibitors of PCR were removed. Cellular digests from Papanicolaou-stained cervical smears did not yield products from PCR, whereas cellular digests from unstained cervical smears always yielded PCR products. Analysis of individual Papanicolaou stain reagents revealed inhibition of PCR by hematoxylin and aluminum sulfate. These inhibitors could be removed from Papanicolaou-stained cervical smears by destaining the slides with 1% HCl. The hematoxylin and aluminum sulfate within Papanicolaou stain inhibit PCR but can be removed from Papanicolaou-stained cytologic specimens using a simple destaining p...Continue Reading


Feb 1, 2005·The Journal of Molecular Diagnostics : JMD·Kathleen M MurphyJames R Eshleman
Nov 26, 2002·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·M E CruickshankH C Kitchener
Feb 12, 1998·Thyroid : Official Journal of the American Thyroid Association·Y TakiyamaM A Zeiger
Jun 1, 2001·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·M T SiddiquiM Saji
Jun 8, 2001·American Journal of Clinical Pathology·B AvidorM Giladi
Oct 1, 2004·Cytopathology : Official Journal of the British Society for Clinical Cytology·K CanfellV Beral
Feb 7, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Deepa J BhandareVictor V Levenson Chernokhvostov
Jun 23, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Hironori TakagiMasao Hirose
Mar 4, 2000·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·T MuraseT Eimoto
Jun 13, 2000·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Y ManF A Tavassoli
Nov 8, 2002·Cytopathology : Official Journal of the British Society for Clinical Cytology·Amina E L HamidiMing-Qing Du
May 7, 2020·Histochemistry and Cell Biology·Alexandra PoteAna Marques-Ramos
Jun 14, 2000·Plastic and Reconstructive Surgery·I G Kara, F Fişekçi
Mar 22, 2014·Molecular and Clinical Oncology·Paola UliviDino Amadori

Related Concepts

Aluminum hydrogen sulfate
Alum Compounds
DNA, Double-Stranded
Staining and Labeling
Pap Smear
Nested Polymerase Chain Reaction
Papanicolaou Test

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Related Papers

Cytopathology : Official Journal of the British Society for Clinical Cytology
J J O'LearyR Chetty
Diagnostic Cytopathology
Lori A HaackSuzanne M Selvaggi
© 2021 Meta ULC. All rights reserved