PMID: 22574523May 12, 2012Paper

Inhibitory activity of fermented milk with Lactobacillus casei strain Shirota against common multidrug-resistant bacteria causing hospital-acquired infections

Journal of the Medical Association of Thailand = Chotmaihet Thangphaet
Surapee Tiengrim, Visanu Thamlikitkul

Abstract

To determine inhibitory activity of fermented milk with Lactobacillus casei strain Shirota (FMLC) against common multi-drug-resistant (MDR) bacteria causing hospital-acquired infections. Time-kill methods of FMLC and cell-free filtered fluid of FMLC (CF-FMLC) against Acinetobacter baumannii, Pseudomonas aeruginosa, ESBL-producing Escherichia coli & Klebsiella pneumoniae and methicillin-resistant Staphylococcus aureus were conducted. The control solutions were Mueller Hinton broth (MHB) and distilled water. The mixtures of FMLC, CF-FMLC, MHB and DW with 10(5) to 10(6) CFU/ml of each bacterium were prepared and incubated at 35 degrees C. Each mixture was quantified for viable bacteria at 0, 1, 3, 6 and 24 hr after incubation onto brain heart infusion agar plates. The inoculated agar plates were incubated at 35 degrees C for 24-48 hr. Bacterial colonies on agar plates were counted and compared among the mixtures. Log CFUs of each organism in MHB and distilled water after incubation were increased from 5.1-6.3 at 0 h to 6.4- > 11 at 24 hr. Log CFUs of each organism in FMLC and CF-FMLC after incubation with study bacteria for 0, 1, 3, 6 and 24 hr were decreased to undetectable amounts at 24 hr. FMLC and CF-FMLC exerted slow inhibito...Continue Reading

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