Inhibitory and excitatory axon terminals share a common nano-architecture of their Cav2.1 (P/Q-type) Ca(2+) channels

Frontiers in Cellular Neuroscience
Daniel AlthofAkos Kulik

Abstract

Tuning of the time course and strength of inhibitory and excitatory neurotransmitter release is fundamental for the precise operation of cortical network activity and is controlled by Ca(2+) influx into presynaptic terminals through the high voltage-activated P/Q-type Ca(2+) (Cav2.1) channels. Proper channel-mediated Ca(2+)-signaling critically depends on the topographical arrangement of the channels in the presynaptic membrane. Here, we used high-resolution SDS-digested freeze-fracture replica immunoelectron microscopy together with automatized computational analysis of Cav2.1 immunogold labeling to determine the precise subcellular organization of Cav2.1 channels in both inhibitory and excitatory terminals. Immunoparticles labeling the pore-forming α1 subunit of Cav2.1 channels were enriched over the active zone of the boutons with the number of channels (3-62) correlated with the area of the synaptic membrane. Detailed analysis showed that Cav2.1 channels are non-uniformly distributed over the presynaptic membrane specialization where they are arranged in clusters of an average five channels per cluster covering a mean area with a diameter of about 70 nm. Importantly, clustered arrangement and cluster properties did not show...Continue Reading

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Citations

Feb 19, 2016·Channels·Martin HeineArthur Bikbaev
Jan 24, 2018·Journal of Neurophysiology·Rozita LaghaeiMarkus Dittrich
Jun 14, 2017·Proceedings of the National Academy of Sciences of the United States of America·Takafumi MikiAlain Marty
Apr 19, 2020·The Journal of Physiology·Tamara RadulovicSamuel M Young
May 17, 2017·Proceedings of the National Academy of Sciences of the United States of America·Cristina E ConstantinBernd Fakler
Nov 12, 2017·Scientific Reports·Miklos SzoboszlayZoltan Nusser
Jan 17, 2019·Nature Reviews. Neuroscience·Jeremy S Dittman, Timothy A Ryan
Apr 1, 2021·Journal of the American Society of Nephrology : JASN·Marlies SachsCatherine Meyer-Schwesinger
Nov 3, 2021·Physiological Reviews·Carolin Wichmann, Thomas Kuner

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Methods Mentioned

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electron microscopy

Software Mentioned

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