Abstract
To study how soluble citrullinated telopeptides of type I and II collagens inhibit the binding of autoantibodies to the respective antigens immobilized onto solid phase, and to use modifications of previous methods to re-analyse rheumatoid arthritis (RA) and control serum samples. Autoantibody binding was inhibited with normal or citrullinated carboxytelopeptides using enzyme-linked immunosorbent assay (ELISA) methods. Serum samples were available from 120 patients with RA and 81 controls. Autoantibodies that bind normal C-telopeptides were not inhibited with soluble normal or citrullinated telopeptides. However, the antibodies that bind only citrullinated telopeptides could be inhibited with corresponding citrullinated telopeptides. Thus, it is not necessary to study the binding of autoantibodies to normal collagens if the specificity of their binding is assessed by immunological inhibition. For type I telopeptide, there are two arginines, the latter of which, when citrullinated, is important for binding. For type II telopeptide, there is one arginine, which is important when citrullinated. The other amino acids, e.g. the last alanine, have only a slight effect on binding. These improved methods differentiate better between RA...Continue Reading
References
Jul 14, 1979·British Medical Journal·B J YoungT J Hamblin
Feb 1, 1986·Arthritis and Rheumatism·M RowleyB Phillips
Feb 1, 1981·Annals of the Rheumatic Diseases·R B ClagueP J Holt
Feb 14, 1998·The Journal of Clinical Investigation·G A SchellekensW J van Venrooij
Jan 22, 2000·Arthritis and Rheumatism·G A SchellekensW J van Venrooij
Jul 1, 1964·Annals of the Rheumatic Diseases·R L NIENHUIS, E MANDEMA
Oct 15, 2003·Trends in Molecular Medicine·Jane Worthington, Sally John
Oct 15, 2003·Arthritis and Rheumatism·Solbritt Rantapää-DahlqvistWalther J van Venrooij
Oct 28, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Erik R VossenaarGer J M Pruijn
Nov 8, 2003·Trends in Molecular Medicine·Ryo YamadaKazuhiko Yamamoto
Feb 12, 2004·Arthritis and Rheumatism·Markus M J NielenBen A C Dijkmans
Mar 17, 2004·Annals of the Rheumatic Diseases·E R VossenaarW J van Venrooij
Mar 17, 2004·Arthritis and Rheumatism·F A van GaalenT W J Huizinga
Nov 9, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Suzanne NijenhuisWalther J vanVenrooij
Dec 3, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Ryo YamadaKazuhiko Yamamoto
Apr 16, 2005·European Journal of Immunology·Harald BurkhardtRikard Holmdahl
May 17, 2005·Autoimmunity Reviews·Ryo Yamada
May 19, 2005·Arthritis Research & Therapy·Karin LundbergHelena Erlandsson Harris
Jun 14, 2005·Biochemical and Biophysical Research Communications·Akari SuzukiKazuhiko Yamamoto
Aug 18, 2005·Rheumatology·X ChangK Yamamoto
Sep 16, 2005·Annals of the Rheumatic Diseases·M-K KoivulaJ Risteli
Nov 29, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Marja-Kaisa KoivulaJuha Risteli
Citations
May 3, 2007·Annals of the Rheumatic Diseases·Marja-Kaisa KoivulaJuha Risteli
Sep 26, 2013·Journal of Translational Medicine·Sanna TurunenJuha Risteli
Mar 1, 2007·Expert Review of Clinical Immunology·Juha RisteliLeila Risteli
Jul 10, 2010·Arthritis and Rheumatism·Sanna TurunenJuha Risteli
Nov 13, 2014·Clinical Chemistry and Laboratory Medicine : CCLM·Jere HäyrynenMarja-Kaisa Koivula
Jan 13, 2015·Arthritis Research & Therapy·Sanna TurunenJuha Risteli
Apr 22, 2016·Autoimmunity·Sanna TurunenJuha Risteli