Inhibitory effect of Abrus abrin-derived peptide fraction against Dalton's lymphoma ascites model

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Sujit K BhutiaTapas K Maiti

Abstract

Peptides derived from larger molecules that are important modulators in cancer regression are becoming leads for development of therapeutic drugs. It has been reported that Abrus abrin, isolated from the seeds of Abrus precatorius, showed in vitro and in vivo antitumor properties by the induction of apoptosis. The present study was designed to evaluate the in vivo therapeutic effectiveness of abrin-derived peptide (ABP) fraction in Dalton's lymphoma (DL) mice model. The lethal dose (LD(50)) of ABP was found to be 2.25 mg/kg body weight and further the acute toxicity was determined with sublethal doses in normal mice. The acute toxicity like body weight, peripheral blood cell count, lympho-hematological and biochemical parameters remained unaffected till 200 microg/kg body weight of ABP. The sublethal doses of ABP showed very significant growth inhibitory properties in vivo DL mice model. There were 24%, 70.8% and 89.7% reductions in DL cell survival in 25, 50 and 100 microg/kg body weight of ABP, respectively. Analysis of the growth inhibitory mechanism in DL cells revealed nuclear fragmentation, and condensation with the appearance of the sub-G(0)/G(1) peak is indicative of apoptosis. Further, the Western blotting showed that ...Continue Reading

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Citations

Jul 30, 2014·Life Sciences·Prashanta Kumar PandaSujit K Bhutia
Mar 24, 2009·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Sujit K BhutiaTapas K Maiti
Oct 1, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Yu-Huei LiuJung-Yaw Lin
May 9, 2009·Cell Biology International·Sujit K BhutiaTapas K Maiti

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