Inhibitory effect of anethole on T-lymphocyte proliferation and interleukin-2 production through down-regulation of the NF-AT and AP-1

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Sung Su YeaChul-Ho Yun

Abstract

Anethole is a naturally occurring alkenylbenzene found in a variety of foods and essential oils. In the present study, we investigated the effect of anethole on T-cell function and the regulatory mechanism of its effect. Direct addition of anethole to B6C3F1 mouse splenocyte cultures produced a concentration-dependent inhibition of the lymphoproliferative response to concanavalin A stimulation. Anethole inhibited phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io)-induced interleukin-2 (IL-2) mRNA expression and protein secretion in EL4 mouse T-cells as determined by quantitative/competitive RT-PCR and ELISA, respectively. To further characterize the mechanism for the transcriptional regulation of IL-2, an electrophoretic mobility shift assay was performed to evaluate the binding activity of the nuclear factor of activated T-cells (NF-AT), activator protein-1 (AP-1), nuclear factor-kappaB (NF-kappaB), and octamer binding protein (Oct) in PMA/Io-stimulated EL4 cells. Anethole decreased the NF-AT and AP-1 binding activity, but no significant effect was observed on NF-kappaB or Oct binding activity. These results suggest that anethole suppress T-cell proliferation and IL-2 production and that the inhibition is mediated, at l...Continue Reading

References

Oct 28, 1988·Science·P A Baeuerle, D Baltimore
Jan 1, 1980·Immunological Reviews·K A Smith
Jun 1, 1995·Current Opinion in Immunology·J JainA Rao
Jan 1, 1997·Annual Review of Immunology·A RaoP G Hogan
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·H NakanoK Okumura
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·L LingD V Goeddel
Sep 25, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P NewberneR A Ford
Mar 23, 2001·Nature Immunology·S K Dower
Apr 21, 2001·Toxicology and Applied Pharmacology·S SiebenB Blömeke
Apr 21, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S K Abraham
Jun 13, 2001·Oncogene·F MaciánA Rao

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Citations

Jun 1, 2011·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Masatoshi TakeiriKazuo Umezawa
Dec 20, 2012·Naunyn-Schmiedeberg's Archives of Pharmacology·Talita Perdigão DomicianoCiomar Aparecida Bersani-Amado
Mar 21, 2009·Journal of Cellular Biochemistry·Vincent Kam Wai WongLiang Liu
Aug 26, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Francielli Maria de Souza Silva-ComarRoberto Kenji Nakamura Cuman
Jun 25, 2016·The Journal of Physical Chemistry. B·Camilla CalabreseWalther Caminati
Aug 9, 2016·Journal of Forensic and Legal Medicine·Hasan GökceSumeyra Demirkiran
Oct 23, 2012·Pharmacological Reports : PR·Edson L PonteAna M S Assreuy
Jun 18, 2020·International Journal of Molecular Sciences·Paweł KwiatkowskiBarbara Dołęgowska
Jan 1, 2012·Biomolecules & Therapeutics·Je Hyeong LeeHyun Pyo Kim
May 31, 2018·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·N A R SáJ R Figueiredo

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