PMID: 19916923Nov 18, 2009Paper

Inhibitory effect of angiostatins on activity of the plasminogen/plasminogen activator system

Biochemistry. Biokhimii︠a︡
R B AĭsinaS D Varfolomeyev

Abstract

Angiostatins, kringle-containing fragments of plasminogen, are potent inhibitors of angiogenesis. Effects of three angiostatin forms, K1-3, K1-4, and K1-4.5 (0-2 microM), on the rate of native Glu-plasminogen activation by its physiological activators in the absence or presence of soluble fibrin were investigated in vitro. Angiostatins did not affect the intrinsic amidolytic activities of plasmin and plasminogen activators of tissue type (tPA) and urokinase type (single-chain scuPA and two-chain tcuPA), but inhibited conversion of plasminogen to plasmin in a dose-dependent manner. All three angiostatins suppressed Glu-plasminogen activation by tcuPA independently of the presence of fibrin, and the inhibitory effect increased in the order: K1-3 < K1-4 < K1-4.5. The inhibitory effects of angiostatins on the scuPA activator activity were lower and further decreased in the presence of fibrin. Angiostatin K1-3 (up to 2 microM) had no effect, while 2 microM angiostatins K1-4 and K1-4.5 inhibited the fibrin-stimulated Glu-plasminogen activation by tPA by 50 and 100%, respectively. The difference in effects of the three angiostatins on the Glu-plasminogen activation by scuPA, tcuPA, and tPA in the absence or presence of fibrin is due t...Continue Reading

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Citations

Nov 20, 2014·Bulletin of Experimental Biology and Medicine·I P ShabalkinM V Gudkova
Mar 13, 2015·Biomedit︠s︡inskai︠a︡ khimii︠a︡·A A TykhomyrovT V Grinenko

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