Inhibitory effect of azelastine hydrochloride on synthesis and release of platelet activating factor from human alveolar macrophages

Prostaglandins, Leukotrienes, and Essential Fatty Acids
K ShindoM Fukumura

Abstract

The effect of azelastine hydrochloride (azelastine) on synthesis and release of platelet activating factor (PAF) in alveolar macrophages obtained from asthmatic and non-asthmatic subjects was examined. Alveolar macrophages (AMs) were preincubated with or without azelastine and stimulated with f-Met-Leu-Phe (fMLP, 10 microM) for 15 min. PAF activity was detected by aggregation of washed guinea pig platelets. PAF activity released from alveolar macrophages (AMs) from asthmatics without preincubation of azelastine was 15.97 [2.17] (mean [SD], ng/10(7) cells) in supernatants and 42.52 [10.16] in cell pellets. After preincubation with 10(-8), 10(-6), and 10(-4) M of azelastine, PAF activity reduced to 10.71 [2.73] (mean [SD], ng/10(7) cells), 7.86 [0.94], and 3.52 [0.31] in the supernatants, and 35.58 [7.37], 21.57 [4.36], and 14.77 [0.99] (n = 15) in the cell pellets, respectively. PAF activity in non-asthmatic subjects without preincubation of azelastine was 8.55 [1.16] (mean [SD], ng/10(7) cells) in supernatants and 32.64 [3.37] in cell pellets. After preincubation with 10(-8), 10(-6), and 10(-4) M of azelastine, PAF activity reduced to 6.68 [0.78] (mean [SD], ng/10(7) cells), 4.47 [0.51], and 2.97 [0.36] in the supernatants, and...Continue Reading

References

Aug 1, 1992·The American Review of Respiratory Disease·E ScappaticciG Camussi
Jan 1, 1985·International Archives of Allergy and Applied Immunology·N ChandR D Sofia
Feb 1, 1986·Journal of Applied Physiology·A O Fels, Z A Cohn
Dec 1, 1986·The Journal of Clinical Investigation·A J WardlawA B Kay
Oct 1, 1986·Agents and Actions·J Morley
Nov 1, 1988·The American Review of Respiratory Disease·M S BalterM Peters-Golden
Jan 1, 1985·Methods of Biochemical Analysis·D J Hanahan, S T Weintraub
Feb 1, 1983·The Journal of Clinical Investigation·M JosephJ Benveniste
Mar 1, 1984·The Journal of Allergy and Clinical Immunology·D A FieldsN Chand
May 1, 1994·American Journal of Respiratory and Critical Care Medicine·D P SpenceS T Holgate
Oct 1, 1996·Prostaglandins, Leukotrienes, and Essential Fatty Acids·K Shindo, M Fukumura
May 1, 1997·Prostaglandins, Leukotrienes, and Essential Fatty Acids·K ShindoK Koide
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

❮ Previous
Next ❯

Citations

Mar 29, 2001·Drugs·G M WalshH Timmerman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.