PMID: 9431460Feb 12, 1998Paper

Inhibitory effect of dideoxyforskolin on cell death induced by ricin, modeccin, diphtheria toxin, and Pseudomonas toxin in MDCK cells

Cell Structure and Function
T OdaT Muramatsu

Abstract

We have found that 1,9-Dideoxyforskolin (DDF) strongly inhibited the cell death induced by ricin, modeccin, Pseudomonas toxin, and diphtheria toxin in MDCK cells, suggesting that these protein toxins have a DDF-sensitive common pathway leading to cell death. However, no significant effect of forskolin on these toxins was observed, implying that cAMP-independent DDF specific mechanism is responsible for the inhibitory effect. The protective effect of DDF against ricin-induced cell death was significantly reversed by the increase in extracellular Ca2+ concentrations. The addition of brefeldin A (BFA) also reversed the protective effect of DDF, while BFA alone slightly increased the cytotoxicity of ricin. The protein synthesis inhibitory activity of modeccin was strongly inhibited by DDF, while only partial inhibition of the activities of ricin and diphtheria toxin was observed. However, the activity of Pseudomonas toxin was enhanced by DDF rather than inhibited. Thus, the process leading to cell death and protein synthesis inhibition by these toxins may be separately affected by DDF, and the protective effect of DDF against toxin-induced cell death is distinct from its effect on protein synthesis inhibition by toxins. Forskolin a...Continue Reading

Citations

Oct 7, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Hiroki AndoYasuo Hatate
Apr 8, 1999·Bioscience, Biotechnology, and Biochemistry·T OdaT Muramatsu
May 31, 2002·Bioscience, Biotechnology, and Biochemistry·Ai KageyamaTsuyoshi Muramatsu
Jun 21, 2019·Toxins·Natalia Sowa-RogozińskaMonika Słomińska-Wojewódzka
Jun 5, 2002·Toxicology Letters·Tadashi TamuraTsuyoshi Muramatsu

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