PMID: 8589501Aug 1, 1995Paper

Inhibitory effect of forskolin on myosin phosphorylation-dependent and independent contractions in bovine tracheal smooth muscle

Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi
M TajimiH Karaki

Abstract

In bovine tracheal smooth muscle, carbachol (CCh, 1 microM) and high K+ (72.7 mM) induced sustained increases in cytosolic Ca2+ level ([Ca2+]i), myosin light chain (MLC) phosphorylation and force of contraction. Forskolin (FK, 1-10 microM) inhibited the CCh-induced increase in [Ca2+]i, MLC phosphorylation and force in parallel. In contrast, FK inhibited the high K(+)-induced contraction and MLC phosphorylation without changing [Ca2+]i. In the absence of extracellular Ca2+ (with 0.5 mM EGTA), CCh (10 microM) and caffeine (20 mM) induced transient increase in [Ca2+]i and contractile force by releasing Ca2+ from cellular store. FK strongly inhibited the CCh-induced Ca2+ transient, but failed to inhibit the caffeine-induced Ca2+ transient. In the absence of external Ca2+, 12-deoxyphorbol 13-isobutylate (DPB, 1 microM) induced sustained contraction without increase in [Ca2+]i and MLC phosphorylation. FK inhibited this contraction without changing [Ca2+]i. In permeabilized muscle, Ca2+ induced contraction in a concentration-dependent manner. FK (10 microM) and cAMP (1-100 microM) shifted the Ca(2+)-force curve to the higher Ca2+ levels. CCh with GTP, GTP gamma S or DPB enhanced contraction in the presence of constant level of Ca2+. F...Continue Reading

Citations

Oct 24, 2003·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Takeharu KanedaShinjiro Nakajyo
Jul 1, 2005·Journal of Pharmacological Sciences·Takeharu KanedaShinjiro Nakajyo
Aug 2, 2007·Journal of Pharmacological Sciences·Takeharu KanedaKazumasa Shimizu
Oct 30, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hai-Ying ZhangJoseph N Benoit
Feb 14, 2006·Journal of Pharmacological Sciences·Takeharu KanedaRyo Hondo

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