Inhibitory effect of human hydrosalpingeal fluid on mouse preimplantation embryonic development is significantly reduced by the addition of lactate

Human Reproduction
C A MurrayS L Tan

Abstract

Implantation and pregnancy rates following in-vitro fertilization-embryo transfer are reduced in the presence of hydrosalpinges, but the basis of the inhibition is unknown. We examined the effect of hydrosalpingeal fluid on preimplantation development of mouse embryos. Embryos cultured in 100% hydrosalpingeal fluid were significantly inhibited developmentally as compared to embryos cultured in 100% defined medium, which served as controls. In contrast, embryos cultured in 50% hydrosalpingeal fluid/50% defined medium reached the blastocyst stage at the same frequency as the controls. When lactate (final concentration 10 mM) was added to 100% hydrosalpingeal fluid, the percentage of cultured embryos that reached the blastocyst stage was significantly increased as compared to 100% hydrosalpingeal fluid, although the percentage was slightly lower than that observed for embryos cultured in the 100% defined medium. A similar but less pronounced effect occurred when pyruvate was added to hydrosalpingeal fluid. These results do not support the concept that a potent embryotoxic agent is commonly present in hydrosalpingeal fluid. Rather, they are consistent with the notion that the inhibitory effect of hydrosalpingeal fluid on embryonic ...Continue Reading

Citations

Jul 19, 2005·Journal of Assisted Reproduction and Genetics·Mohamed A BedaiwyAshok Agarwal
Nov 6, 2004·Journal of Assisted Reproduction and Genetics·Annika StrandellAnn Wallin
Aug 24, 2010·Human Reproduction Update·Hakan Cakmak, Hugh S Taylor
Aug 23, 2000·Current Opinion in Obstetrics & Gynecology·R MansourG I Serour
May 7, 2004·Current Opinion in Obstetrics & Gynecology·Pinar H KodamanEmre Seli
Jun 25, 2005·Current Opinion in Obstetrics & Gynecology·Luca Sabatini, Colin Davis
Jul 13, 2007·Current Opinion in Obstetrics & Gynecology·Annika Strandell
Nov 28, 2001·The Journal of Obstetrics and Gynaecology Research·L C AjonumaC J Haines
Mar 1, 2002·Human Reproduction·Mohamed A BedaiwyRakesh Sharma
Feb 20, 2007·Annals of the New York Academy of Sciences·John N Bontis, Theodoros D Theodoridis
Nov 5, 2016·Journal of Ovarian Research·Marco NoventaStefano Palomba
Mar 30, 2001·Human Reproduction·C de Vantéry ArrighiD Chardonnens
Feb 12, 2005·Human Reproduction·Louis Chukwuemeka AjonumaHsiao Chang Chan
Aug 24, 1999·Biology of Reproduction·S J DowningH J Leese

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