Inhibitory effect of lamotrigine on A-type potassium current in hippocampal neuron-derived H19-7 cells

Epilepsia
Chin-Wei HuangSheng-Nan Wu

Abstract

We investigated the effects of lamotrigine (LTG) on the rapidly inactivating A-type K+ current (IA) in embryonal hippocampal neurons. The whole-cell configuration of the patch-clamp technique was applied to investigate the ion currents in cultured hippocampal neuron-derived H19-7 cells in the presence of LTG. Effects of various related compounds on IA in H19-7 cells were compared. LTG (30 microM-3 mM) caused a reversible reduction in the amplitude of IA. The median inhibitory concentration (IC50) value required for the inhibition of IA by LTG was 160 microM. 4-Aminopyridine (1 mM), quinidine (30 microM), and capsaicin (30 microM) were effective in suppressing the amplitude of IA, whereas tetraethylammonium chloride (1 mM) and gabapentin (100 microM) had no effect on it. The time course for the inactivation of IA was changed to the biexponential process during cell exposure to LTG (100 microM). LTG (300 microM) could shift the steady-state inactivation of IA to a more negative membrane potential by approximately -10 mV, although it had no effect on the slope of the inactivation curve. Moreover, LTG (100 microM) produced a significant prolongation in the recovery of IA inactivation. Therefore in addition to the inhibition of volt...Continue Reading

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Citations

Jul 8, 2005·Psychopharmacology·Charles H LargeDonald C Goff
Jan 3, 2007·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Brian S Meldrum, Michael A Rogawski
Nov 24, 2005·Epilepsia·Athanasios Covanis
Jun 11, 2005·Epilepsia·Andrew G HerzogAndrew S Blum
Mar 4, 2008·British Journal of Pharmacology·C-H LeeH-H Liou
Apr 6, 2007·Journal of Neuroscience Research·Chin-Wei HuangSheng-Nan Wu
Feb 18, 2005·Epilepsy Research·Bengt R DanielssonTorbjörn Tomson
Dec 1, 2005·Brain : a Journal of Neurology·Stefan Remy, Heinz Beck
May 21, 2010·Expert Opinion on Pharmacotherapy·Tanvir U Syed, Martha Sajatovic

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