PMID: 11897996Mar 19, 2002Paper

Inhibitory effect of lidocaine on T cells from patients with allergic asthma

The Journal of Allergy and Clinical Immunology
Akihiko TanakaMitsuru Adachi

Abstract

Nebulized lidocaine enables dosages of oral corticosteroids to be tapered in the treatment of severe asthma. We sought to investigate the effect of lidocaine on T cells from patients with allergic asthma. PBMCs and CD4(+) T cells were isolated from 6 patients with asthma and house dust mite allergy. PBMCs were cultured with lidocaine for up to 7 days and then stained with propidium iodide to evaluate the involvement of apoptosis. In addition, the viability of CD4(+) T cells when cultured with lidocaine was investigated. Effects of lidocaine on proliferative response, mRNA expression, and protein production of IL-5 and IFN-gamma by PBMCs were investigated after stimulation with Dermatophagoides farinae, purified protein derivative, and phorbol 12-myristate 13-acetate plus calcium ionophore. The effects of lidocaine on the proliferative response of steroid-insensitive PBMCs from 6 nonallergic donors induced by preculture with IL-2 and IL-4 were also investigated. No significant increase in the staining of PBMCs with propidium iodide was observed in the presence of 100 micromol/L lidocaine. The viability of CD4(+) T cells was not significantly affected by culture with lidocaine at this concentration. However, lidocaine inhibited, ...Continue Reading

Citations

May 8, 2009·The Journal of Veterinary Medical Science·Takaaki AndoDaisaku Watanabe
Sep 30, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P C OlsenM A Martins
Feb 9, 2013·Journal of Feline Medicine and Surgery·Laura A NafeCarol R Reinero
Apr 22, 2004·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Robert HinesSteven Prakken
Apr 25, 2006·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K T R SamsonM Adachi
Sep 24, 2004·Current Medical Research and Opinion·Bradley S GalerArnold R Gammaitoni
Jun 2, 2011·Case Reports in Medicine·Akiyuki UzawaSatoshi Kuwabara
Apr 30, 2017·Clinical and Experimental Otorhinolaryngology·Hwan Soo KimHyun Hee Kim
Jun 18, 2005·Memórias do Instituto Oswaldo Cruz·Rodrigo A SiqueiraMarco A Martins
Apr 17, 2004·Der Anaesthesist·S PecherM W Hollmann
Jul 28, 2005·Allergologia et immunopathologia·S de Paz ArranzA Pérez Pimiento

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