Inhibitory effect of monogalactosyldiacylglycerol, extracted from spinach using supercritical CO2, on mammalian DNA polymerase activity

Journal of Agricultural and Food Chemistry
Hiroshi IijimaYoshiyuki Mizushina

Abstract

We investigated the effective extraction of monogalactosyldiacylglycerol (MGDG) from dried spinach (Spinacia oleracea) using supercritical fluid carbon dioxide (SC-CO(2)) with a modifier/entrainer. The yield of MGDG in the SC-CO(2) extract was not influenced by increasing temperature at a constant pressure, although the total extract yield was decreased. The total extract yield and MGDG yield in the extract from commercially purchased spinach (unknown subspecies), were greatly influenced by lower pressure. In a modifier (i.e., ethanol) concentration range of 2.5-20%, both the extract and MGDG yield increased as the ethanol concentration rose. The highest total extract yield (69.5 mg/g of spinach) and a good MGDG yield (16.3 mg/g of spinach) were obtained at 80 degrees C, 25 MPa, and 20% ethanol. The highest MGDG concentration (76.0% in the extract) was obtained at 80 degrees C, 25 MPa, and 2.5% ethanol, although the total extract yield under these conditions was low (5.2 mg/g of spinach). The optimal conditions for the extraction of MGDG were 80 degrees C, 20 MPa, and 10% ethanol. Of the 11 subspecies of spinach tested under these conditions, "Ujyou" had the highest concentration of MGDG. The total extract yield and MGDG concen...Continue Reading

References

Apr 19, 1988·Biochemistry·T DateA Matsukage
Dec 1, 1987·Journal of Bioenergetics and Biomembranes·S G Sprague
Jun 5, 2002·Annual Review of Biochemistry·Ulrich HubscherSilvio Spadari
Dec 31, 2002·Biochemical Pharmacology·Chikako MurakamiYoshiyuki Mizushina
Feb 1, 2005·The Journal of Nutritional Biochemistry·Naoki MaedaYoshiyuki Mizushina

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Citations

Jun 9, 2009·Bioscience, Biotechnology, and Biochemistry·Yoshiyuki Mizushina
Jun 17, 2014·Journal of the Science of Food and Agriculture·Laura JaimeGuillermo Reglero
Oct 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Li JiYe Yuan

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