Inhibitory Effect of Opuntia humifusa Fruit Water Extract on Solar Ultraviolet-Induced MMP-1 Expression

International Journal of Molecular Sciences
Ah-Ram HanChang-Won Cho

Abstract

Opuntia humifusa is a type of cactus whose fruits have been used in folk medicine for the treatment of several diseases. In the present study, we aimed to determine whether O. humifusa fruit water extract (OHE) has inhibitory effects against solar ultraviolet (sUV)-induced matrix metalloproteinase-1 (MMP-1) expression. In ex vivo human skin, we found that OHE suppressed sUV radiation-induced MMP-1 expression. The inhibitory effect of OHE was confirmed in human dermal fibroblasts. OHE treatment reduced sUV-induced MMP-1 expression by suppressing reactive oxygen species (ROS) generation and phosphorylation of c-Jun, a component of transcription factor activator protein 1 (AP-1). On the other hand, OHE recovered the tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) and type 1 collagen production attenuated by sUV. As upstream signaling pathways for AP-1, MKK4-JNK, MEK-ERK, and MKK3/6-p38 phosphorylation were downregulated by OHE treatment. In addition, OHE exhibited DPPH radical scavenging activity. These findings demonstrate that OHE has a preventive effect against sUV-induced skin damage via suppression of pathways triggered by ROS.

References

Jul 14, 1995·The Journal of Biological Chemistry·M Karin
Jan 1, 1996·Pharmacology & Therapeutics·T E Cawston
Nov 14, 1997·The New England Journal of Medicine·G J FisherJ J Voorhees
Sep 5, 2002·Ageing Research Reviews·Laure Rittié, Gary J Fisher
Nov 20, 2002·Archives of Dermatology·Gary J FisherJohn J Voorhees
Jan 6, 2006·The Journal of Pharmacy and Pharmacology·Jae Youl ChoMan-Hee Rhee
May 25, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Jean Magloire FeugangChangping Zou
Jan 30, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ji-Young BaeYoung-Hee Kang
Jan 21, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Lazhar ZorguiWafa Hassen
Jun 23, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·V Gayathri DeviAnnie Abraham
Jan 26, 2011·Journal of the European Academy of Dermatology and Venereology : JEADV·E KohlR-M Szeimies
Nov 26, 2011·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Pulok K MukherjeeBirendra K Sarkar
Apr 17, 2012·Dermatology Research and Practice·Borut Poljšak, Raja Dahmane
May 10, 2013·Journal of Agricultural and Food Chemistry·Diana González-PeñaConcepción Sánchez-Moreno
Nov 8, 2014·Journal of Medicinal Food·Sahng-Wook HahmYong-Suk Son
Feb 6, 2015·Ageing Research Reviews·A Kammeyer, R M Luiten
Apr 24, 2015·Biomolecules·Mark RinnerthalerKlaus Richter
Jan 18, 2017·BMC Complementary and Alternative Medicine·Sung Hee HanHyung Joo Suh

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Citations

Jul 2, 2020·Journal of Medicinal Food·Young-Ran SongTae-Gyu Lim
Dec 15, 2020·Oxidative Medicine and Cellular Longevity·P Veeramani KandanNagarajan Rajendra Prasad

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Methods Mentioned

BETA
ELISA
Protein Assay
electrophoresis
Assay

Software Mentioned

ABION

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