PMID: 29763213Feb 1, 2017Paper

Inhibitory effect of overexpressed kisspeptin on proliferation and migration of MKN-45 gastric cancer cells

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Xin WangZhengmin Wang

Abstract

To investigate the expression of kisspeptin in gastric adenocarcinoma and its effect on the proliferation and migration of gastric cancer cells. The level of kisspeptin in 50 gastric cancer tissues and their paracancerous tissues were detected by quantitative real-time PCR and Western blotting. Kisspeptin-siRNA,control-siRNA,p EGFP-N1-kisspeptin,p EGFP-N1 were separately transfected into MKN-45 gastric cancer cells. After 48-hour culture,the levels of matrix metalloproteinase-2( MMP-2),β-catenin,C-myc,MMP-9,kisspeptin were detected by Western blotting; MTT assay was used to detect the proliferation of MKN-45 cells; wound healing assay was performed to assess the migration ability of MKN-45 cells. After MKN-45 cells were treated with Wnt / β-catenin signal pathway inhibitor FH535,MTT assay and flow cytometry were used to evaluate cell proliferation ability and cell apoptosis,respectively. Kisspeptin expression in gastric adenocarcinoma tissues was significantly lower than that of the adjacent normal tissues. The cell survival rate and migration rate of p EGFP-N1-kisspeptin group were significantly lower than those of p EGFP-N1 group. Cell survival rate and migration rate of kisspeptin-siRNA group were significantly higher than t...Continue Reading

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