Inhibitory effect of periodontally diseased root extracts on the growth of human gingival fibroblasts

Journal of Periodontology
R H OlsonD L Layman

Abstract

Cementum shavings obtained from periodontally diseased and nondiseased areas of 100 removed, single-rooted teeth were extracted with either pyrogen-free water (PFW) for 5 minutes, 1 M citric acid for 5 minutes or 45% phenol-PFW for 90 minutes at 65 degrees C. The extracts were membrane-filtered, dialyzed exhaustively versus PFW, lyophilized, weighed and then dissolved in complete growth medium. The phenol-water or citric acid extracts of cementum shavings from periodontally diseased roots were positive for endotoxin by the limulus lysate assay (LLA). Pyrogen-free water extracts of diseased or phenol-water extracts of nondiseased cementum shavings were negative, or only slightly positive, respectively, for endotoxin by LLA. Media containing the various extracts were added to logarithmically growing cultures of human gingival fibroblasts (HGF). Separate cultures of HGF were exposed to Escherichia coli endotoxin at concentrations of 50, 100, 250 and 500 micrograms/ml to determine the growth-inhibitory effects of a known endotoxin. Cell growth was analyzed by measuring the incorporation of tritiated thymidine into cells. Suppression of HGF growth from 30 to 49% by E. coli endotoxin was concentration-dependent and linear over the co...Continue Reading

References

Dec 1, 1977·Journal of Dental Research·D H FineG D Shockman
Mar 1, 1978·Journal of Periodontal Research·J S GarrettJ Egelberg
Jul 1, 1979·Journal of Periodontology·D Nishimine, T J O'Leary
Nov 1, 1975·Journal of Periodontology·J J AleoP A Farber
Sep 1, 1976·Journal of Periodontology·A A Register, F A Burdick
Sep 1, 1974·Journal of Periodontology·J J AleoA P Varboncoeur
Dec 1, 1972·Journal of Periodontology·M L Morris
Jul 1, 1973·The Journal of Infectious Diseases·J W Shands
Sep 1, 1969·Journal of Dental Research·K A Selvig
Apr 1, 1971·Archives of Oral Biology·C G Hatfield, A Baumhammers
Nov 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·R R Rojas-CoronaJ Fine
Feb 1, 1983·Journal of Periodontology·J A SarbinoffC H Miller
Jan 1, 1980·Journal of Periodontal Research·R T ColeK A Selvig
Feb 1, 1983·Journal of Periodontology·F A DeRenzis, S Y Chen
Apr 1, 1983·Journal of Periodontology·T J O'Leary, A H Kafrawy
Jun 1, 1982·Journal of Periodontology·J M Corley, W J Killoy
Jan 1, 1980·Proceedings of the Society for Experimental Biology and Medicine·A Mcgivney, S G Bradley

❮ Previous
Next ❯

Citations

Jul 1, 1995·Journal of Clinical Periodontology·M Hennequin, Y Douillard
Jan 20, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M Saito, A S Narayana
Jun 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T Yokokoji, A S Narayanan
Oct 1, 1992·Journal of Endodontics·M YamasakiH Nakamura
Jun 4, 2011·Journal of Clinical Periodontology·Giovanpaolo Pini PratoMichele Nieri
May 2, 2009·Journal of Periodontology·Mado G KomboliTheodore A Katsorhis
Jun 25, 2005·Journal of Periodontology·Amer Sayed-SuleymanThomas Lallier
Apr 20, 2004·Journal of Periodontology·Yael Houri-HaddadLior Shapira
Sep 1, 1991·Journal of Dental Research·S OlsonR C Page
Sep 25, 2003·Journal of Endodontics·J J KamburisP D Eleazer
Aug 28, 2004·Periodontology 2000·Patrick A Adriaens, Laurence M Adriaens
Nov 1, 1993·Journal of Periodontal Research·R L MacNeil, M J Somerman
May 1, 1987·Journal of Periodontal Research·J Egelberg
Mar 1, 1990·Journal of Periodontal Research·B McAllisterR C Page
Feb 1, 1996·Journal of Clinical Laser Medicine & Surgery·D SimeoneP Cerisier

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.