Inhibitory Effect of PgAFP and Protective Cultures on Aspergillus parasiticus Growth and Aflatoxins Production on Dry-Fermented Sausage and Cheese

Microorganisms
Josué DelgadoMiguel A Asensio

Abstract

Aflatoxigenic molds can grow and produce aflatoxins on dry-fermented meat and cheese. The small, basic, cysteine-rich antifungal protein PgAFP displays a time-limited inhibitory ability against unwanted molds by increasing reactive oxygen species (ROS), which can lead to increased aflatoxin production. However, calcium abolishes the inhibitory effect of PgAFP on certain Aspergillus spp. To maximize the antifungal effect, this protein may be combined with protective cultures. Yeasts and lactic acid bacteria may counteract the impact of calcium on PgAFP fungal inhibition. The objective of this work was to study the effect of PgAFP and different combined treatments with Debaryomyces hansenii and/or Pediococcus acidilactici against growth of and aflatoxin production by an aflatoxigenic strain of Aspergillus parasiticus in both culture media and dry-fermented foods with low or high calcium levels. Aflatoxins production was increased by PgAFP but dramatically reduced by P. acidilactici in low calcium culture medium, whereas in the Ca-enriched culture medium, all treatments tested led to low aflatoxins levels. To study whether PgAFP and the protective microorganisms interfere with ROS and aflatoxin production, the relative expression ...Continue Reading

References

Jan 1, 1988·The Journal of Applied Bacteriology·J L BlancoG Suárez
Apr 1, 1996·International Journal of Food Microbiology·F NúñezM A Asensio
Nov 21, 2000·Free Radical Biology & Medicine·T Jayashree, C Subramanyam
May 22, 2001·Environmental Health Perspectives·J D Miller
Sep 8, 2001·International Journal of Food Microbiology·M H TaniwakiG H Fleet
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 12, 2003·Archives of Microbiology·Lydia KaisererFlorentine Marx
Mar 10, 2004·Applied and Environmental Microbiology·Jiujiang YuJoan W Bennett
Apr 20, 2005·Applied Microbiology and Biotechnology·M ReverberiC Fanelli
Sep 2, 2005·Molecular Plant-microbe Interactions : MPMI·Lori A Maggio-HallNancy P Keller
Jan 2, 2008·International Journal of Food Microbiology·Jong H KimBruce C Campbell
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Aug 18, 2009·International Journal of Food Microbiology·Raquel AcostaMiguel A Asensio
Apr 6, 2012·Food Microbiology·Alicia RodríguezJuan J Córdoba
Oct 25, 2012·PloS One·Massimo ReverberiCorrado Fanelli
Feb 21, 2013·Journal of the Science of Food and Agriculture·Vivekananda MandalNarayan Chandra Mandal
Nov 28, 2013·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·C AlapontP V Martínez-Culebras
Dec 3, 2013·International Journal of Food Microbiology·Maria J AndradeLene Jespersen
Oct 4, 2014·International Journal of Food Microbiology·Markus Schmidt-HeydtRolf Geisen
Apr 16, 2015·International Journal of Food Microbiology·Josué DelgadoMiguel A Asensio
Jun 17, 2015·Applied Microbiology and Biotechnology·Josué DelgadoFélix Núñez
Jan 18, 2018·International Journal of Food Microbiology·Belén PeromingoMaría J Andrade
Jul 1, 2014·Comprehensive Reviews in Food Science and Food Safety·Nolwenn HymeryEmmanuel Coton

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Methods Mentioned

BETA
PCR

Software Mentioned

Primer
- Blast

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An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.