PMID: 9450668Feb 5, 1998Paper

Inhibitory effect of regucalcin on Ca2+/calmodulin-dependent protein kinase activity in rat renal cortex cytosol

Molecular and Cellular Biochemistry
H Kurota, M Yamaguchi

Abstract

The effect of regucalcin on Ca2+/calmodulin-dependent protein kinase activity in the cytosol of rat renal cortex was investigated. Regucalcin is a calcium-binding protein which exists in rat liver and renal cortex. Protein kinase activity in renal cortex cytosol was markedly increased by the addition of CaCl2 (0.5 mM) plus calmodulin (10 microg/ml) in the enzyme reaction mixture . This increase was completely prevented by the addition of trifluoperazine (25 microM), an antagonist of calmodulin. The cytosolic Ca2+/calmodulin-dependent protein kinase activity was clearly inhibited by the addition of regucalcin; an appreciable effect of regucalcin was seen at 0.01 microM. The cytosolic Ca2+/calmodulin-dependent protein kinase activity was fairly increased by increasing concentrations of added Ca2+ (100-1000 microM). This increase was markedly blocked by the presence of regucalcin (0.1 microM). The inhibitory effect of regucalcin on the protein kinase activity was also seen with varying concentrations of calmodulin (2-20 microg/ml). These results demonstrate that regucalcin can regulate Ca2+/calmodulin-dependent protein kinase activity in renal cortex cells.

Citations

Mar 24, 2005·Journal of Cellular Biochemistry·Taeko Nakagawa, Masayoshi Yamaguchi
Mar 23, 2013·Cellular and Molecular Life Sciences : CMLS·Ricardo MarquesSílvia Socorro
Mar 25, 2011·Molecular and Cellular Biochemistry·Masayoshi Yamaguchi
Jun 2, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Masayoshi Yamaguchi
Sep 24, 2015·International Journal of Molecular Medicine·Masayoshi Yamaguchi
Aug 14, 2001·Journal of Cellular Biochemistry·Y Morooka, M Yamaguchi
Sep 28, 2000·Biochemical and Biophysical Research Communications·M Yamaguchi
May 16, 2000·Life Sciences·M Yamaguchi
Apr 11, 2003·Journal of the Neurological Sciences·Minako Tobisawa, Masayoshi Yamaguchi

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