Inhibitory effect of αS1- and αS2-casein hydrolysates on angiotensin I-converting enzyme in human endothelial cells in vitro, rat aortic tissue ex vivo, and renovascular hypertensive rats in vivo

Journal of Dairy Science
D Rousseau-RalliardA Grynberg

Abstract

A great number of milk-derived peptides have been shown to exhibit angiotensin converting enzyme (ACE) inhibitory properties and thus potential utility in the regulation of blood pressure. The present work aimed to investigate the effects of 2 milk trypsin hydrolysates from alpha(S1)- and alpha(S2)-casein (CH1 and CH2, respectively) on ACE activity evaluated in human umbilical vein endothelial cells (HUVEC) in vitro, rat aortic tissues ex vivo, and renovascular hypertensive rat in vivo. Incubation of HUVEC and rat aortic tissues with CH1 or CH2 induced a concentration-dependent inhibition of hydrolysis of the ACE substrate hippuryl-histidyl-leucine (HHL), the hydrolysates being much less potent than perindopril (an ACE inhibitor). However, in contrast to perindopril, CH1 and CH2 failed to modify angiotensin I-induced aortic ring vasoconstriction. The HPLC profiles of rat plasma after intragastric administration were variable among individuals but none of the observed peaks corresponded to peptides comprising CH1 or CH2 or to fragments of these peptides. During 4 wk of cardiovascular monitoring, in hydrolysate-fed renovascular hypertensive rats, systolic blood pressure weakly decreased compared with the control group. However, t...Continue Reading

References

Jan 1, 1988·Annual Review of Nutrition·M L Gardner
Jul 1, 1971·Biochemical Pharmacology·D W Cushman, H S Cheung
Aug 1, 1995·Bioscience, Biotechnology, and Biochemistry·H MatsufujiY Osajima
Dec 1, 1993·Journal of Hypertension. Supplement : Official Journal of the International Society of Hypertension·M A BennettH Thurston
Sep 1, 1995·Journal of Cardiovascular Pharmacology·D J CampbellS B Harrap
May 23, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·E BechgaardH W Nielsen
Jan 1, 1997·Biopolymers·N Yamamoto
Aug 27, 1998·In Vitro Cellular & Developmental Biology. Animal·I V BalyasnikovaA B Fisher
Jan 14, 1999·Journal of Hypertension·G M LondonM E Safar
Nov 22, 2001·Molecular and Cellular Biochemistry·D RousseauA Grynberg
Jan 11, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Delphine RousseauAlain Grynberg
Jan 24, 2003·The American Journal of Clinical Nutrition·Leena SeppoRiitta Korpela
May 29, 2003·Current Pharmaceutical Design·H Meisel, R J FitzGerald
Mar 1, 1956·The Journal of Experimental Medicine·L T SKEGGSN P SHUMWAY
Dec 25, 2003·Journal of Enzyme Inhibition and Medicinal Chemistry·Anders FuglsangNiels C B Nyborg
Feb 26, 2004·Journal of Biochemical and Biophysical Methods·Anders FuglsangNiels C B Nyborg
Aug 4, 2004·American Journal of Hypertension·Shunsaku MizushimaHirotsugu Ueshima
Oct 8, 2004·The British Journal of Nutrition·Vanessa VermeirssenWilly Verstraete
Mar 16, 2006·Journal of Dairy Science·P J HuthG D Miller
Jul 20, 2006·Journal of Agricultural and Food Chemistry·José M CentenoPaloma Manzanares
Jan 3, 2007·American Journal of Hypertension·Jenny A CadéeChin-Kun Wang
Sep 23, 2008·The Journal of Nutrition·Vanessa Robbez MassonDelphine Rousseau
Jan 1, 1948·The American Journal of Medicine·H GOLDBLATT

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Citations

Jul 19, 2014·Critical Reviews in Food Science and Nutrition·Ahmed S M SalehQun Shen
Feb 3, 2015·Molecular Nutrition & Food Research·Marine S Da Silva, Iwona Rudkowska
Jan 27, 2018·Applied Biochemistry and Biotechnology·Mara Rose de OliveiraEduardo Basílio de Oliveira
Dec 23, 2010·Journal of Agricultural and Food Chemistry·Griet HerregodsGuy Smagghe

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