Inhibitory effect of Sihoga-Yonggol-Moryo-Tang on matrix metalloproteinase-2 and -9 activities and invasiveness potential of hepatocellular carcinoma

Pharmacological Research : the Official Journal of the Italian Pharmacological Society
Ki-Tae HaCheorl-Ho Kim

Abstract

A Korean traditional herbal formulation, Sihoga-Yonggol-Moryo-Tang (SGYMT), is being used for the treatment of many diseases such as tumor, neuropsychiatric, and vascular disorders. The extracts prepared from SGYMT and its herbal ingredients were assayed for the inhibitory effects on tumor-specific matrix metalloproteinases-2 and -9 (MMP-2/9) activities using gelatin zymography. The results showed that SGYMT decreased the gelatinolytic activities of MMP-2 and -9, which were secreted from SK-Hep1 cells. The IC(50) values of SGYMT against these enzymes were 85 and 145 micrograms ml(-1), respectively. The cytotoxocities of SGYMT and its ingredients on human hepatocellular carcinoma (HCC) cell, SK-Hep1 cells, were very low (IC(50)>150 micrograms ml(-1)) as measured by the XTT assay method. The extract were then investigated the inhibitory effect on the invasion of SK-Hep1 cells using matrigel precoated transwell chambers. The results showed that SGYMT effectively inhibited the invasion of SK-Hep1 cells as compared with the control groups. In addition, among herbal ingredients of SGYMT, the bark of Cinnamomum cassia BLUME (Cinnamomi Cortex) and roots of Scutellaria baicalensis GEORGI (Scutelleria Radix) have shown significant inhibi...Continue Reading

Citations

Jan 5, 2011·Biochemistry Research International·Noel Vinay Thomas, Se-Kwon Kim
Jul 30, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Yan Li, Robert C G Martin
Oct 19, 2011·Environmental Toxicology and Pharmacology·Noel Vinay Thomas, Se-Kwon Kim
Jan 13, 2010·Journal of Ethnopharmacology·Xiaofei ShangZhengping Jia
Apr 15, 2008·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Subo WangXianjun Qu
May 2, 2014·Environmental Toxicology and Pharmacology·Noel Vinay ThomasSe-Kwon Kim
Nov 28, 2013·Integrative Cancer Therapies·Seong Woo YoonBharat B Aggarwal

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.