Inhibitory effect of the compounds from the water extract of Curcuma longa on the production of PGE2 and NO in a macrophage cell line stimulated by LPS

Bioscience, Biotechnology, and Biochemistry
Kengo KawasakiYoshihiro Yamamoto

Abstract

We wished to search for the compounds contributing to the anti-inflammatory effects of the water extract of Curcuma longa (WEC). WEC was fractioned and the fractions were evaluated with regard to their inhibitory effect on the production of nitric oxide (NO) from the macrophage cell line stimulated by lipopolysaccharide. Compounds in the active fractions were isolated and identified. One isolated compound was identified as new: (6S)-2-methyl-5-hydroxy-6-(3-hydroxy-4-methylphenyl)-2-heptene-4-one (1). Four isolated compounds were identified as known: (6S)-2-methyl-6-(4-hydroxyphenyl)-2-heptene-4-one (4), bisabolone-4-one (5), curcumenone (6), and turmeronol A (8). Three isolated compounds were not identified their stereostructures but their planar structures: 2-methyl-6-(4-hydroxymethyl-phenyl)-2-heptene-4-one (2), 2-methyl-6-(2,3-epoxy-4-methyl-4-cyclohexene)-2-heptene (3), and 4-methylene-5-hydroxybisabola-2,10-diene-9-one (7). Compounds 1, 4, 7 and 8 inhibited production of prostaglandin E2 and NO. Others inhibited NO production only. These results (at least in part) show the active compounds contributing to the anti-inflammatory effects of WEC, and may be useful for elucidating its various beneficial physiologic effects.

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Citations

Sep 12, 2019·BMC Complementary and Alternative Medicine·Wendy Pearson, Laima S Kott
Feb 16, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Amanda Mara TelesFernando Almeida-Souza
Nov 17, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Kong Yen LiewChau Ling Tham

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Methods Mentioned

BETA
nuclear magnetic resonance
enzyme-linked immunosorbent assay

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