Inhibitory effect of the gut microbial linoleic acid metabolites, 10-oxo-trans-11-octadecenoic acid and 10-hydroxy-cis-12-octadecenoic acid, on BV-2 microglial cell activation

Journal of Pharmacological Sciences
Shiori IkeguchiToshiaki Kume

Abstract

10-oxo-trans-11-octadecenoic acid (KetoC) and 10-hydroxy-cis-12-octadecenoic acid (HYA) are long-chain fatty acids generated from linoleic acid by the gut lactic acid bacterium Lactobacillus plantarum. These fatty acids have been reported to have anti-inflammatory activity in the intestine. However, little is known about their effects in the brain. In this study, we aimed to investigate the effects of these fatty acids on lipopolysaccharide (LPS)-induced inflammatory processes in mouse microglial cells (BV-2 cells). KetoC and HYA inhibited LPS-induced nitric oxide (NO) production and suppressed the expression of inducible NO synthase in BV-2 cells. NO changes in these inhibitory effects were observed with AH7614, a G-protein coupled receptor 120 antagonist, or the peroxisome proliferator-activated receptors antagonists, GW6471 and GW9662. In addition, KetoC and HYA did not inhibit translocation of p65, a subunit of NF-κB, or IκB degradation. Similarly, no effect on p38 or JNK phosphorylation was observed. However, KetoC and HYA were found to inhibit ERK phosphorylation induced by LPS, suggesting that these fatty acids may exert their anti-inflammatory effects through the inhibition of ERK activation in microglial cells.

Citations

Mar 3, 2020·Gut Microbes·Bo Yang
Nov 20, 2019·Inflammation·Amanda N CareyBarbara Shukitt-Hale
Jul 17, 2020·Frontiers in Microbiology·Susan M WernimontJan S Suchodolski
May 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Benso SulijayaKazuhisa Yamazaki
Jun 27, 2019·Neuroscience Letters·Q LeyrolleA Nadjar
May 6, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Jignesh Mohanbhai SoniSurajit Karmakar
Nov 25, 2021·Microbiology Spectrum·Christopher D RadkaCharles O Rock

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