Inhibitory Effect of Vaginal Lactobacillus Supernatants on Cervical Cancer Cells

Probiotics and Antimicrobial Proteins
Ke-Di WangJian-Rong Su

Abstract

Lactobacilli have been shown to inhibit the proliferation of several types of cancer cells, but the effects of vaginal Lactobacilli on cervical cancer cells have seldom been reported. We incubated Caski cells with supernatants of predominant strains in the vagina and investigated their effects on cell growth and the possible mechanisms. Cell-free supernatants of Lactobacillus crispatus, L. jensenii, and L. gasseri were prepared and purified. Caski cells were treated with various concentrations of Lactobacillus supernatants (LS). The effect of LS on cell growth was investigated using MTT assays. The influence of LS on the cell cycle and expression of human papillomavirus (HPV) E6 and E7 oncogenes was determined by flow cytometry and RT-PCR, respectively. LS-inhibited Caski cell proliferation caused morphological changes in a pH-independent manner. Flow cytometric analysis revealed that cells exposed to LS exhibited a significant increase of cell number in S phase and a strong decrease of cell number in G2/M phase. Expression of HPV E6 and E7 oncogenes, as well as CDK2 and cyclin A was decreased after treatment with LS, while expression of p21 was increased. Supernatants of L. crispatus, L. jensenii, and L. gasseri have inhibitor...Continue Reading

References

Apr 1, 1993·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·A G Ostör
Dec 3, 1999·Applied and Environmental Microbiology·V S OcañaM E Nader-Macías
Jun 24, 2000·Microbes and Infection·S Boris, C Barbés
Nov 9, 2000·Proceedings of the National Academy of Sciences of the United States of America·E C Goodwin, D DiMaio
Nov 25, 2000·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·N Muñoz
Aug 24, 2001·American Journal of Obstetrics and Gynecology·A AroutchevaS Faro
Mar 29, 2002·Journal of Clinical Pathology·F X BoschK V Shah
May 28, 2009·Journal of Gynecologic Oncology·Ka Hyun NamYong Wook Jung
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jacques RavelLarry J Forney
Sep 8, 2010·Carcinogenesis·Enrique BoccardoLuisa L Villa
Apr 19, 2011·Colloids and Surfaces. B, Biointerfaces·Amy McMillanGregor Reid
May 12, 2011·Biochemical and Biophysical Research Communications·Pengcheng LiQian Yang
May 19, 2011·Future Microbiology·Rachel R Spurbeck, Cindy Grove Arvidson
Aug 27, 2011·Infectious Diseases in Obstetrics and Gynecology·Caroline C KingJack D Sobel
Dec 30, 2011·Journal of Clinical Microbiology·Shigeru FujimuraMitsuji Kaji
Aug 30, 2012·International Journal of STD & AIDS·Y-L GuoL Geng
Jan 16, 2013·Therapeutic Advances in Gastroenterology·Peera Hemarajata, James Versalovic
Jan 29, 2013·BMC Infectious Diseases·Sonal PendharkarHarold Marcotte

❮ Previous
Next ❯

Citations

May 21, 2020·Infection and Drug Resistance·Yuanyue LiZulqarnain Baloch
Jun 13, 2020·Journal of Ovarian Research·Moghaddaseh JahanshahiShahla Chaichian
Feb 12, 2021·Frontiers in Cellular and Infection Microbiology·Fenghao ZhangTingtao Chen
Jul 10, 2019·Food Research International·Muhammad Shahid Riaz RajokaJunling Shi
Jun 3, 2021·Microorganisms·Melanie C Alonzo MartínezVicente Navarro-López
Jun 15, 2021·Current Oncology Reports·Laura M ChambersChad Michener
Jul 25, 2021·Seminars in Cancer Biology·Mohd WahidRamandeep Rattan
Oct 3, 2021·Probiotics and Antimicrobial Proteins·Shiyue HuHongpeng He
Oct 5, 2021·Frontiers in Cellular and Infection Microbiology·Ningxia SunWen Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.