PMID: 25744831Mar 7, 2015Paper

Inhibitory effect of wogonin on the proliferation and invasion of glioblastoma U87 cells and related mechanism

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Zhiqing Zeng, Hong Liu

Abstract

To explore the effect of wogonin on the proliferation and invasion of glioblastoma U87 cells and its related mechanism. Glioblastoma U87 cells were cultured in vitro in RPMI1640 medium added with 100 mL/L fetal bovine serum. After cell adherence, the cells were inoculated with 20 μL culture solution (control group), 20 μL wogonin solutions (0, 50 and 100) μmol/L for 48 hours, respectively. The 0 μmol/L wogonin group used 20 μL PBS instead. Cell proliferation was analyzed by MTT assay. The cell invasion ability was detected using Transwell™ invasion assay. The expressions of ezrin mRNA was examined through real-time quantitative PCR. The expressions of ezrin, Bcl-2 and Bax proteins as well as phosphorylated ezrin protein level were measured by Western blotting. Apoptosis index (AI) was determined through terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay. The inhibitory rate of cell proliferation significantly increased in 50 and 100 μmol/L wogonin groups as compared with 0 μmol/L wogonin group and control group. Moreover, that was higher in 100 μmol/L wogonin group than 50 μmol/L wogonin group. In comparison with control and 0 μmol/L wogonin groups, the mean cell numbers of ...Continue Reading

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