Inhibitory effect on the tobacco mosaic virus infection by a plant RING finger protein

Virus Research
Yasuyuki YamajiTadaaki Hibi

Abstract

In the yeast two-hybrid screening of plant factors interacting with tobacco mosaic virus (TMV) RNA-dependent RNA polymerase (RdRp), we found a protein containing a RING finger motif in tobacco (Nicotiana tabacum) and designated it as TARF (TMV-associated RING finger protein). TARF is a homologue of a Lotus japonicus RING finger protein (LjnsRING) involved in the symbiotic interaction between L. japonicus and Mesorhizobium loti. When TARF was silenced by virus-induced gene silencing (VIGS) method, TMV RNA accumulation as well as the number of foci formed by GFP-tagged TMV increased drastically. Transient overexpression of TARF reduced the accumulation of TMV. Moreover, TARF transcription was rapidly upregulated by the inoculation of TMV in tobacco plants. These results indicated that TARF is a RING finger protein that inhibits the accumulation of TMV via the interaction of TMV RdRp.

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Citations

Nov 21, 2013·Nature Communications·Takanori KobayashiNaoko K Nishizawa
May 5, 2016·Plant Molecular Biology·Takanori KobayashiNaoko K Nishizawa
Mar 1, 2019·Frontiers in Plant Science·Jorge Rodríguez-CelmaJanneke Balk
Jul 10, 2019·Molecular Plant Pathology·Hernan Garcia-Ruiz
Jul 18, 2012·Plant Physiology·Catherine Alcaide-Loridan, Isabelle Jupin

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