Inhibitory effects and underlying mechanism of 7-hydroxyflavone phosphate ester in HeLa cells.

PloS One
Ting ZhangQi Jin

Abstract

Chrysin and its phosphate ester have previously been shown to inhibit cell proliferation and induce apoptosis in Hela cells; however, the underlying mechanism remains to be characterized. In the present study, we therefore synthesized diethyl flavon-7-yl phosphate (FP, C(19)H(19)O(6)P) by a simplified Atheron-Todd reaction, and explored its anti-tumor characteristics and mechanisms. Cell proliferation, cell cycle progression and apoptosis were measured by MTS, flow cytometry and terminal deoxynucleotidyl transferase dUTP nick end labeling techniques, respectively in human cervical cancer HeLa cells treated with 7-hydroxyflavone (HF) and FP. p21, proliferating cell nuclear antigen (PCNA) and cAMP levels in Hela cells were analyzed by western blot and radioimmunoassay. Both HF and FP inhibited proliferation and induced apoptosis in HeLa cells via induction of PCNA/p21 expression, cleaved caspase-3/poly (ADP-ribose) polymerase (PARP)-1, elevation of cAMP levels, and cell cycle arrest with accumulation of cells in the G0/G1 fraction. The effects of FP were more potent than those of HF. The interactions of FP with Ca(2+)-calmodulin (CaM) and Ca(2+)-CaM-phosphodiesterase (PDE)1 were explored by electrospray ionization-mass spectromet...Continue Reading

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Citations

Oct 24, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Katrin Sak
May 17, 2015·Endocrine-related Cancer·Rodrigo B de AlexandreFabio R Faucz
Nov 9, 2017·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Monika StomporJoanna Wietrzyk

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Methods Mentioned

BETA
fluorescence spectrometry
flow cytometry
Assay
electrophoresis

Software Mentioned

Odyssey infrared
Modfit

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