Inhibitory effects of (-)-epigallocatechin-3-gallate and pterostilbene on pancreatic cancer growth in vitro

The Journal of Surgical Research
Shannon F KostinDavid McFadden

Abstract

It has been previously shown that the naturally occurring antioxidant (-)-epigallocatechin-3-gallate (EGCG), found in green tea, and pterostilbene, a stilbenoid derived from blueberries, inhibit pancreatic cancer in vitro when used individually. We hypothesized that the combination of EGCG and pterostilbene would reveal additive effects in vitro. Using the pancreatic cancer cell lines MIA PaCa-2 and PANC-1, efficacy and synergism were evaluated for cell proliferation and viability (3-(4,5-dimethyltiazol-2-y1)-2,5-diphenltetrazolium bromide assays, cell cycle analysis) and mitochondrial apoptosis (mitochondrial depolarization, cytochrome C release, caspase-3/7 activity, cell death detection using enzyme-linked immunosorbent assay). Cell proliferation assays revealed significant additive antiproliferative effects with pterostilbene and EGCG in both cell lines at the later, 72-h, point (P < 0.05). MIA underwent S-phase arrest with the combination (10-12% increase); however, cell cycle arrest was not observed in PANC. The combination induced mitochondrial depolarization and upregulated cytochrome C (P < 0.05) in MIA, but these effects were not observed in PANC. EGCG increased caspase-3/7 in MIA; however, the combination did not sig...Continue Reading

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Citations

Nov 1, 2012·Cancer Letters·Srinivas Reddy Boreddy, Sanjay K Srivastava
May 22, 2013·Oxidative Medicine and Cellular Longevity·Denise McCormack, David McFadden
Sep 16, 2015·The Journal of Physical Chemistry. B·Dominic BottenCarla Molteni
Jul 3, 2013·Critical Reviews in Clinical Laboratory Sciences·José M EstrelaMiguel Asensi
Sep 21, 2016·Critical Reviews in Food Science and Nutrition·Ren-You GanHarold Corke
Jun 22, 2017·Molecular Informatics·Emiliano LaudadioRoberta Galeazzi
Sep 22, 2019·International Journal of Molecular Sciences·Itika AroraTrygve O Tollefsbol
Apr 7, 2018·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Masome Ghasemi-PirbalutiPezhman Beshkar
Apr 20, 2018·Future Medicinal Chemistry·Weimei ShiLonghuo Wu
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Oct 4, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Xing Liu, Yongyi Bi
Feb 18, 2020·Journal of Agricultural and Food Chemistry·Wei-Sheng LinMin-Hsiung Pan

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